REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.
Biotechnol J. 2013 Jun;8(6):671-81. doi: 10.1002/biot.201200328. Epub 2013 May 6.
Polymer monoliths are an efficient platform for antibody purification. The use of monoclonal antibodies (mAbs) and engineered antibody structures as therapeutics has increased exponentially over the past few decades. Several approaches use polymer monoliths to purify large quantities of antibody with defined clinical and performance requirements. Functional monolithic supports have attracted a great deal of attention as they offer practical advantages for antibody purification, such as more rapid analysis, smaller sample volume requirements and the opportunity for a greater target molecule enrichment. This review focuses on the development of synthetic and natural polymer-based monoliths for antibody purification. The materials and methods employed in monolith production are discussed, highlighting the properties of each system. We also review the structural characterization techniques available using monolithic systems and their performance under different chromatographic approaches to antibody capture and release. Finally, a summary of monolithic platforms developed for antibody separation is presented, as well as expected trends in research to solve current and future challenges in this field. This review comprises a comprehensive analysis of proposed solutions highlighting the remarkable potential of monolithic platforms.
聚合物整体柱是一种有效的抗体纯化平台。在过去几十年中，单克隆抗体（mAbs）和工程化抗体结构作为治疗药物的应用呈指数级增长。有几种方法使用聚合物整体柱来纯化具有明确临床和性能要求的大量抗体。功能整体柱因其在抗体纯化方面具有实际优势而引起了广泛关注，例如更快速的分析、更小的样品体积要求以及更大的目标分子富集机会。本综述重点介绍了用于抗体纯化的合成和天然聚合物基整体柱的开发。讨论了整体柱生产中使用的材料和方法，强调了每个系统的特性。我们还综述了可用的整体柱系统的结构特征技术及其在不同的抗体捕获和释放色谱方法下的性能。最后，介绍了为抗体分离开发的整体平台的总结，以及解决该领域当前和未来挑战的预期研究趋势。本综述对提出的解决方案进行了全面分析，突出了整体平台的巨大潜力。
