Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi 830011, Xinjiang, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Sep 1;934:109-16. doi: 10.1016/j.jchromb.2013.07.006. Epub 2013 Jul 15.
One of the main challenges in MIPs preparation is the proper MIP monolith design for water-soluble compounds due to the difficulty in satisfying the demands of both good column permeability and affinity to polar template. A new strategy of metallic pivot in a ternary porogenic system of dimethyl sulfoxide (DMSO)-dimethylformamide (DMF)-1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4) was suggested to solve this problem. An imprinted monolithic column with high porosity and good permeability was synthesized using a mixture of methyl gallate (template), 4-vinylpyridine, ethylene glycol dimethacrylate, and cobalt acetate. Some polymerization factors, such as template-monomer molar ratio and the composition of the ionic liquid, on the imprinting effect of the resulting MIPs monoliths were systematically investigated. In a mobile phase of acetonitrile-buffer, the greatest imprinting factor of 10.9 was obtained on the MIPs monolith with the optimized polymerization parameters. Thermodynamic analysis for separation demonstrated that the separation between the template and its analogs on the ion-mediated MIPs monolith is an enthalpy-controlled process.
在分子印迹聚合物（MIPs）的制备中，主要挑战之一是针对水溶性化合物进行合适的 MIP 整体设计，因为难以同时满足良好的柱渗透性和对极性模板的亲和力这两个要求。本研究提出了在二甲亚砜（DMSO）-二甲基甲酰胺（DMF）-1-丁基-3-甲基咪唑四氟硼酸盐（[Bmim]BF4）三元致孔体系中使用金属枢轴的新策略，以解决这一问题。使用没食子酸甲酯（模板）、4-乙烯基吡啶、乙二醇二甲基丙烯酸酯和乙酸钴的混合物合成了具有高孔隙率和良好渗透性的印迹整体柱。系统研究了聚合过程中的一些因素，如模板-单体摩尔比和离子液体组成，对所得 MIP 整体柱的印迹效果的影响。在乙腈-缓冲液的流动相中，在优化的聚合参数下，MIP 整体柱的最大印迹因子为 10.9。分离的热力学分析表明，离子介导的 MIPs 整体柱上模板与其类似物之间的分离是一个焓控制过程。
