Zhang Xianghui, Fu Jianming, Hiromasa Yasuaki, Pan Hongyu, Bai Guihua
Jinlin University, Changchun, Jilin, People's Republic of China ; Department of Plant Pathology, Kansas State University, Manhattan, Kansas, United States of America.
United States Department of Agriculture/Agricultural Service, Hard Winter Wheat Genetics Research Unit, Kansas State University, Manhattan, Kansas, United States of America.
PLoS One. 2013 Dec 20;8(12):e82079. doi: 10.1371/journal.pone.0082079. eCollection 2013.
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, substantially reduces wheat grain yield and quality worldwide. Proteins play important roles in defense against the fungal infection. This study characterized differentially expressed proteins between near-isogenic lines (NILs) contrasting in alleles of Fhb1, a major FHB resistance gene in wheat, to identify proteins underlining FHB resistance of Fhb1.
The two-dimensional protein profiles were compared between the Fusarium-inoculated spikes of the two NILs collected 72 h after inoculation. The protein profiles of mock- and Fusarium-inoculated Fhb1(+) NIL were also compared to identify pathogen-responsive proteins.
Eight proteins were either induced or upregulated in inoculated Fhb1(+) NIL when compared with mock-inoculated Fhb1(+) NIL; nine proteins were either induced or upregulated in the Fusarium-inoculated Fhb1(+) NIL when compared with Fusarium-inoculated Fhb1(-) NIL. Proteins that were differentially expressed in the Fhb1(+) NIL, not in the Fhb1(-) NIL, after Fusarium inoculation included wheat proteins for defending fungal penetration, photosynthesis, energy metabolism, and detoxification.
Coordinated expression of the identified proteins resulted in FHB resistance in Fhb1(+) NIL. The results provide insight into the pathway of Fhb1-mediated FHB resistance.
小麦赤霉病（FHB）主要由禾谷镰刀菌引起，在全球范围内大幅降低了小麦的籽粒产量和品质。蛋白质在抵御真菌感染中发挥着重要作用。本研究对小麦主要抗赤霉病基因Fhb1等位基因不同的近等基因系（NILs）之间差异表达的蛋白质进行了表征，以鉴定Fhb1介导的赤霉病抗性相关蛋白。
比较接种镰刀菌72小时后收集的两个NILs接种穗的二维蛋白质谱。还比较了模拟接种和接种镰刀菌的Fhb1(+) NIL的蛋白质谱，以鉴定病原体响应蛋白。
与模拟接种的Fhb1(+) NIL相比，接种的Fhb1(+) NIL中有8种蛋白质被诱导或上调；与接种镰刀菌的Fhb1(-) NIL相比，接种镰刀菌的Fhb1(+) NIL中有9种蛋白质被诱导或上调。接种镰刀菌后，在Fhb1(+) NIL中差异表达而在Fhb1(-) NIL中未差异表达的蛋白质包括用于抵御真菌穿透、光合作用、能量代谢和解毒的小麦蛋白。
所鉴定蛋白质的协同表达导致Fhb1(+) NIL具有抗赤霉病能力。这些结果为Fhb1介导的赤霉病抗性途径提供了见解。
