Defence Institute of High Altitude Research, Defence Research and Development Organisation, Leh-Ladakh, Jammu and Kashmir, India.
Defence Institute of Physiology and Allied Sciences, Defence Research and Development Organisation, Timarpur, Delhi, India.
PLoS One. 2013 Dec 23;8(12):e83008. doi: 10.1371/journal.pone.0083008. eCollection 2013.
We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca), and roseroot (Rhodiola imbricata) from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS). Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC) with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID) with fatty acid methyl esters (FAMEs) derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA) and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs), palmitoleic acid (43.47%), oleic acid (20.89%), and linoleic acid (4.31%) were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES), and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit in the stressful environment and could be used as a high value nutritional supplement.
我们评估了来自喜玛拉雅山脉的沙棘（Hippophae rhamnoides）、杏（Prunus armeniaca）和玫瑰根（Rhodiola imbricata）植物鸡尾酒的营养价值、维生素含量、氨基酸组成、脂肪酸含量和矿物质组成。通过快速分辨液相色谱/串联质谱（RRLC-MS/MS）测定植物鸡尾酒中的游离维生素形式。检测到维生素 E 和 B 族维生素是主要的维生素。采用反相高效液相色谱（RP-HPLC）和柱前衍生化法鉴定和定量氨基酸。定量了 8 种必需氨基酸和 11 种非必需氨基酸，含量范围为 76.33-9485.67μg/g。在必需氨基酸中，L-蛋氨酸、L-苯丙氨酸、L-赖氨酸、L-亮氨酸和 L-组氨酸含量较高。我们还通过脂肪酸甲酯（FAMEs）衍生化气相色谱结合火焰电离检测器（GC-FID）定量了植物鸡尾酒中的脂肪酸。分析表明，4 种主要脂肪酸贡献了总脂质含量。棕榈酸是饱和脂肪酸（SFA）的丰富来源，占总脂质含量的约 31%。在不饱和脂肪酸（UFA）中，棕榈油酸（43.47%）、油酸（20.89%）和亚油酸（4.31%）含量较高。通过电感耦合等离子体发射光谱仪（ICP-OES）进行矿物质分析，发现含有多种重要的膳食矿物质元素。印度喜玛拉雅寒冷沙漠高海拔地区气候恶劣、地形复杂、物流条件有限，导致新鲜水果和蔬菜稀缺。因此，这种植物鸡尾酒中的多种维生素、必需氨基酸、脂肪酸和膳食矿物质来源将为人们在高压力环境下提供巨大的健康益处，并可作为高价值营养补充剂使用。
