Defence Institute of High Altitude Research, Defence Research & Development Organisation, C/o 56 APO, Leh-Ladakh, Jammu & Kashmir, Pin 901205, India.
BMC Complement Altern Med. 2013 Oct 7;13:259. doi: 10.1186/1472-6882-13-259.
Himalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca) and roseroot (Rhodiola imbricata) from trans-Himalaya.
The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and nitric oxide (NO) radical scavenging capacities and lipid peroxidation inhibition (LPI) property of the extracts were determined. Total antioxidant power was determined by ferric reducing/antioxidant power (FRAP) assay. Total polyphenol, flavonoid, flavonol, proanthocyanidin and carotenoid were also estimated for both extracts. We have identified and quantified the phyto-chemotypes present in the methanol and n-hexane extracts by hyphenated gas chromatography/mass spectrometry (GC/MS) technique.
Antioxidant capacity assays using DPPH, ABTS, NO, LPI and FRAP exhibited analogous results where the phytococktail showed high antioxidant action. The phytococktail was also found to possess high quantity of total polyphenol, flavonoid, flavonol and carotenoid. A significant and linear correlation was found between the antioxidant capacities and bioactive principles. A total of 32 phyto-chemotypes were identified from these extracts by GC/MS chemometric fingerprinting. Major phyto-chemotypes identified by GC/MS were glycosides, phenylpropanoids and derivatives, terpenoids, alkaloids, phytosterols, fatty acids and esters, alkaloids and derivatives, organic acid esters and aromatic ethers with positive biological and pharmacological actions.
The phytococktail extracts were found to contain considerable amount of diverse bioactive compounds with high antioxidant capacities. The presence of hydrophilic and lipophilic antioxidants in the phytococktail could have contributed to the higher antioxidant values. Hence, the phytococktail could be used as natural source of antioxidants to ameliorate disorders associated with oxidative stress.
喜马拉雅地区的植物在传统医学中被广泛应用，既可作为预防药物，也可作为治疗高山疾病的药物。我们的目的是评估由沙棘（Hippophae rhamnoides）、杏（Prunus armeniaca）和玫瑰根（Rhodiola imbricata）组成的植物鸡尾酒的甲醇和正己烷提取物的抗氧化能力和生物活性化合物。
测定提取物的 1,1-二苯基-2-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）和一氧化氮（NO）自由基清除能力以及脂质过氧化抑制（LPI）特性。总抗氧化能力通过铁还原/抗氧化能力（FRAP）测定法确定。还对两种提取物的总多酚、类黄酮、黄酮醇、原花青素和类胡萝卜素进行了估计。我们通过联用气相色谱/质谱（GC/MS）技术鉴定并定量了甲醇和正己烷提取物中的植物化学型。
使用 DPPH、ABTS、NO、LPI 和 FRAP 进行的抗氧化能力测定显示，植物鸡尾酒表现出高抗氧化作用。植物鸡尾酒还含有大量的总多酚、类黄酮、黄酮醇和类胡萝卜素。抗氧化能力和生物活性成分之间存在显著的线性相关性。通过 GC/MS 化学计量指纹图谱从这些提取物中鉴定出 32 种植物化学型。通过 GC/MS 鉴定出的主要植物化学型为糖苷、苯丙素及其衍生物、萜类、生物碱、植物甾醇、脂肪酸和酯、生物碱及其衍生物、有机酸酯和芳香醚，具有积极的生物和药理学作用。
植物鸡尾酒提取物含有相当数量的具有高抗氧化能力的不同生物活性化合物。植物鸡尾酒中亲水性和疏水性抗氧化剂的存在可能促成了更高的抗氧化值。因此，植物鸡尾酒可作为抗氧化剂的天然来源，用于改善与氧化应激相关的疾病。
