Parfenov V N, Galaktionov K I
Tsitologiia. 1987 Feb;29(2):142-9.
For identification and distribution of actin microfilaments in hand-isolated nuclei of R. temporaria oocytes (stage 6, according to Dumont, 1972) different methods were used: heavy meromyosin decoration, antiactin immunofluorescence with monoclonal antibodies, staining with rhodamine phalloidin, and electrophoresis in polyacrylamide gel. The nuclei of R. temporaria oocytes contain a considerable quantities of actin microfilaments which form intranuclear meshwork. Microfilaments are connected with the nucleoli, nucleolar RNP-complexes and nuclear envelope. Immunofluorescence with antiactin monoclonal antibodies reveals a strong staining of microfilaments and nucleoli. A slight staining of nucleoli is observed after the treatment of nuclei with rhodamine phalloidin. A specific role of intranuclear microfilaments in direct transport of nucleolar material from the nucleus into the oocyte cytoplasm, in stabilization of the karyosphere (the late diplotene oocyte complex of chromosomes with numerous nucleoli) is discussed in addition to its keeping in a definite region of the nucleus. A supposition is drawn on the functional significance of the connection between microfilaments and nuclear matrix. Based on our own and literature data, a conclusion is drawn, that the intranuclear filament actin may be one of the leading components in morpho-functional organization of the nucleus as the whole.
为了鉴定并分布欧洲林蛙卵母细胞（根据杜蒙特1972年的分类为6期）手工分离细胞核中的肌动蛋白微丝，使用了不同的方法：重酶解肌球蛋白标记法、用单克隆抗体进行抗肌动蛋白免疫荧光法、用罗丹明鬼笔环肽染色法以及聚丙烯酰胺凝胶电泳法。欧洲林蛙卵母细胞核含有大量形成核内网的肌动蛋白微丝。微丝与核仁、核仁核糖核蛋白复合体以及核膜相连。用抗肌动蛋白单克隆抗体进行免疫荧光显示微丝和核仁有强烈染色。用罗丹明鬼笔环肽处理细胞核后，观察到核仁有轻微染色。除了将核内微丝保持在细胞核的特定区域外，还讨论了其在将核仁物质从细胞核直接运输到卵母细胞胞质中、稳定核球（具有众多核仁的双线期晚期卵母细胞染色体复合体）方面的特定作用。对微丝与核基质之间连接的功能意义进行了推测。根据我们自己的数据和文献资料得出结论：核内丝状肌动蛋白可能是整个细胞核形态功能组织中的主要成分之一。
