Pochukalina G N, Tsarfenov V N
Tsitologiia. 1994;36(11):1027-34.
The oocytes of the 6th stages, according to Dumont (1972), taken from R. temporaria in nature in April were studied. The semithin sections of Araldit-embedded material, as well as preparations of karyospheres with capsules isolated from nuclei using Callan's method (Callan et al., 1987), were analysed. Ultrastructural investigations were carried out both on intact nuclei and on nuclei extracted in 2M NaCl. The April oocytes differ from the winter ones previously analysed by Gruzova and Parfenov (1977), in the distribution of capsule components: the fibrous material surrounds, as previously, the central part of karyosphere, whereas the pseudomembranes penetrate into the chromosome tangle. The fibrous material reveals stability (resistance) to 2M NaCl treatment. Another feature of the April oocytes is the development of small intracellular bodies (0.3-2 microns). These bodies are composed of coiled threads 40-50 nm in diameter and connected with the nucleoli. The morphogenesis of the capsule fibrous component is discussed in connection with the double nature of the capsule material. Participation of actin filaments in the formation of the fibrillar part of the capsule is discussed. A similarity of the bodies composed of coiled threads in oocyte nuclei is drawn with the "coiled bodies" of somatic cell (Lamond, Carmo-Fonseca, 1993).
根据杜蒙特（1972年）的研究，对4月份从自然界采集的林蛙第六期卵母细胞进行了研究。分析了用环氧树脂包埋材料制作的半薄切片，以及用卡伦方法（卡伦等人，1987年）从细胞核中分离出的带包膜核球体标本。对完整细胞核和用2M氯化钠提取的细胞核都进行了超微结构研究。4月份的卵母细胞与格鲁佐娃和帕尔费诺夫（1977年）之前分析的冬季卵母细胞在包膜成分分布上有所不同：纤维物质如之前一样围绕着核球体的中心部分，而假膜则深入到染色体缠结中。纤维物质对2M氯化钠处理具有稳定性（抗性）。4月份卵母细胞的另一个特征是小细胞内体（0.3 - 2微米）的发育。这些小体由直径40 - 50纳米的盘绕细丝组成，并与核仁相连。结合包膜物质的双重性质讨论了包膜纤维成分的形态发生。讨论了肌动蛋白丝在包膜纤维部分形成中的作用。将卵母细胞核中由盘绕细丝组成的小体与体细胞的“卷曲小体”（拉蒙德、卡尔莫 - 丰塞卡，1993年）进行了比较。
