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[小鼠静止和增殖激活肝细胞的细胞核与细胞质中蛋白质和核酸含量的细胞光度分析]

[Cytophotometric analysis of the protein and nucleic acid content of the nucleus and cytoplasm of resting and proliferation-activated hepatocytes in the mouse].

作者信息

Selivanova G V, Vlasova T D, Bogdanova M S

出版信息

Tsitologiia. 1987 Feb;29(2):186-96.

PMID:2437674
Abstract

Mouse hepatocytes, quiescent and activated to proliferation after hepatectomy, were investigated using cytophotometry in the ultraviolet region of selective absorbtion of nucleic acids, and then were examined in the visible region of spectrum after cytochemical reaction for total protein. Some features of RNA and protein accumulation were revealed in the nucleus and cytoplasm of activated cells of different ploidy. The activation resulted in the reduction of histone content and in the increase in the nucleus: cytoplasm ratio in cells of all the ploidy classes.

摘要

利用细胞光度法在核酸选择性吸收的紫外区域对小鼠肝细胞(肝切除术后静止并被激活进入增殖状态)进行了研究,然后在对总蛋白进行细胞化学反应后,在光谱的可见光区域对其进行了检测。在不同倍性的激活细胞的细胞核和细胞质中揭示了RNA和蛋白质积累的一些特征。这种激活导致所有倍性类别的细胞中组蛋白含量降低以及核质比增加。

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Tsitologiia. 1987 Feb;29(2):186-96.
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