Selivanova G V, Nilova V K, Vlasova T D, Bushmarina M S, Rumiantsev P P
Tsitologiia. 1988 Dec;30(12):1454-62.
The cytophotometrical investigation of gallocyanine-chrome alum stained cardiac muscle cells allows to ascertain that a mean content of the nucleic acids calculated for a single nucleus is essentially higher in the left ventricle myocytes in comparison with the left auricle cells of healthy adult rats. These values in 1-, 2- and 3-nuclear cells of the ventricle are, respectively, 21.3, 19.3, and 18.0, and 14.1, 13.7, 13.5 of arbitrary units (a. u.) in the auricle cells. A difference in cytoplasmic RNA contents of the same cells is more significant, these values are 65.7, 116.4, and 158.9 a. u. in ventricle myocytes, and 33.4, 60.8 and 95.2 a. u. in auricle cells. The nucleic acids content in the nuclei and RNA content in the cytoplasm increase with the development of proliferation in myocytes after experimental myocardial infarction. A relative increase in the nucleic acids content in the nuclei of the same cell types reaches 50, 24, and 10% 11 days after infarction and 56, 38, and 45% 31 days after infarction. A relative increase in cytoplasmic RNA of the same cells reaches, respectively, 52, 17, and 25%, and 70, 57, and 53% 11 and 31 days after infarction. These findings evidence on the greatest synthetic activity of the single-nuclear auricle muscle cells in the process of heart restoration after infarction.
对经没食子蓝 - 铬明矾染色的心肌细胞进行细胞光度学研究发现，与健康成年大鼠的左心耳细胞相比，左心室肌细胞单个细胞核中核酸的平均含量显著更高。心室1核、2核和3核细胞中的这些值分别为21.3、19.3和18.0任意单位（a.u.），而心耳细胞中的值分别为14.1、13.7和13.5任意单位（a.u.）。相同细胞的细胞质RNA含量差异更为显著，心室肌细胞中的这些值分别为65.7、116.4和158.9 a.u.，心耳细胞中的值分别为33.4、60.8和95.2 a.u.。实验性心肌梗死后，随着心肌细胞增殖的发展，细胞核中的核酸含量和细胞质中的RNA含量增加。相同细胞类型细胞核中核酸含量的相对增加在梗死后11天达到50%、24%和10%，在梗死后31天达到56%、38%和45%。相同细胞细胞质RNA的相对增加在梗死后11天和31天分别达到52%、17%和25%，以及70%、57%和53%。这些发现证明了单核心耳肌细胞在梗死后心脏恢复过程中具有最大的合成活性。
