Lee Hyo-Seol, Lee Jongeun, Kim Seong-Oh, Song Je-Seon, Lee Jae-Ho, Lee Syng-Ill, Jung Han-Sung, Choi Byung-Jai
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of Korea ; Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
PLoS One. 2013 Dec 23;8(12):e84201. doi: 10.1371/journal.pone.0084201. eCollection 2013.
The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), while in the PDL it was the tumor-suppressor gene WIF1. Genes related to bone development and remodeling (STMN2, IBSP, BMP8A, BGLAP, ACP5, OPN, BMP3, and TM7SF4) and wound healing (IL1, IL8, MMP3, and MMP9) were also more strongly expressed in the PDL than in the dental follicle. In selected genes, a comparison among cDNA microarray, real-time reverse-transcription polymerase chain reaction, and immunohistochemical staining confirmed similar relative gene expressions. The gene-expression profiles presented here identify candidate genes that may enable differentiation between the dental follicle and PDL.
人牙囊部分分化为牙周膜（PDL），但其生物学功能不同。使用cDNA微阵列技术比较牙囊和牙周膜的基因表达谱。微阵列分析鉴定出490个表达差异两倍或更大的基因，其中分别有365个和125个在牙囊和牙周膜中表达更丰富。牙囊中表达最强的基因是那些与骨发育和重塑相关的基因（EGFL6、MMP8、FRZB和NELL1）、细胞凋亡和趋化性相关的基因（Nox4、CXCL13和CCL2）以及牙齿和胚胎发育相关的基因（WNT2、PAX3、FGF7、AMBN、AMTN和SLC4A4），而在牙周膜中表达最强的是肿瘤抑制基因WIF1。与骨发育和重塑相关的基因（STMN2、IBSP、BMP8A、BGLAP、ACP5、OPN、BMP3和TM7SF4）以及伤口愈合相关的基因（IL1、IL8、MMP3和MMP9）在牙周膜中的表达也比牙囊中更强。在选定的基因中，cDNA微阵列、实时逆转录聚合酶链反应和免疫组织化学染色之间的比较证实了相似的相对基因表达。这里呈现的基因表达谱鉴定出了可能有助于区分牙囊和牙周膜的候选基因。
