Stolze B, Kaaden O R
Virology. 1987 May;158(1):174-80. doi: 10.1016/0042-6822(87)90251-0.
It is generally accepted that Aleutian disease virus (ADV) cannot be neutralized by antibodies either in vivo or in vitro. We found several ways to demonstrate neutralization of ADV by specific antibodies from mink. It was essential to make ADV monodisperse by treatment with sodium lauroyl sarkosyl or n-butanol or by filtration through 0.05-micron membranes before neutralization tests. In kinetic experiments, there was a 95% loss of virus infectivity within the first 5 min of reaction, but a resistant fraction of about 1% remained after 1.5 hr of incubation. Neutralization titers between 1:160 and 1:640 were found in sera from naturally and experimentally infected mink. A positive relation was consistently found between neutralization and ELISA titers. Furthermore, separation of phospholipids from ADV was shown by thin-layer chromatography of butanol-extracted virions. By reconstitution of monodispersed ADV with various lipids, phospholipids were found to interfere with virus neutralization by attachment to the virus surface.
一般认为，水貂阿留申病病毒（ADV）在体内或体外均不能被抗体中和。我们发现了几种方法来证明水貂的特异性抗体可中和ADV。在进行中和试验之前，通过用月桂酰肌氨酸钠或正丁醇处理或通过0.05微米膜过滤使ADV单分散是至关重要的。在动力学实验中，反应的前5分钟内病毒感染力损失了95%，但孵育1.5小时后仍有约1%的抗性部分。在自然感染和实验感染的水貂血清中发现中和效价在1:160至1:640之间。中和效价与ELISA效价之间始终存在正相关关系。此外，通过对丁醇提取的病毒粒子进行薄层色谱分析，显示了从ADV中分离出磷脂。通过用各种脂质重构单分散ADV，发现磷脂通过附着在病毒表面而干扰病毒中和。
