Wright P F, Wilkie B N
Am J Vet Res. 1982 May;43(5):865-8.
Experiments were undertaken to investigate the potential of the enzyme-linked immunosorbent assay (ELISA) as a screening test for the diagnosis of the 2 known naturally occurring forms of Aleutian disease of mink. Anti-Aleutian disease virus (ADV) antibody activity was not detectable in the sera of mink with nonprogressive Aleutian disease despite the demonstration of antibody by counterimmunoelectrophoresis (CIEP) in the same sera. Anti-ADV antibody was detectable in 93% of sera from mink at various stages of experimentally induced progressive Aleutian disease. False-negative reactions occurred in sera which demonstrated high anti-ADV antibody titers by CIEP. As a consequence of the high prevalence of false-negative reactions, the ELISA was not considered to be an effective screening test. However, using CIEP as an indicator of ADV infection, the ELISA may be useful in differentiating mink with nonprogressive Aleutian disease from mink with progressive Aleutian disease.
开展了实验以研究酶联免疫吸附测定法（ELISA）作为诊断水貂两种已知自然发生形式的阿留申病的筛查试验的潜力。尽管通过对流免疫电泳（CIEP）在同一血清中证实了抗体，但在非进行性阿留申病水貂的血清中未检测到抗阿留申病病毒（ADV）抗体活性。在实验性诱导的进行性阿留申病不同阶段的水貂血清中，93%可检测到抗ADV抗体。CIEP显示抗ADV抗体滴度高的血清出现假阴性反应。由于假阴性反应的高发生率，ELISA不被认为是一种有效的筛查试验。然而，以CIEP作为ADV感染的指标，ELISA可能有助于区分非进行性阿留申病水貂和进行性阿留申病水貂。
