Ma Chun-Hua, Jiang Rong, Li Jin-Duo, Wang Bin, Sun Li-Wei, Lv Yuan
Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory, Tianjin, China E-mail :
Asian Pac J Cancer Prev. 2014 Jan;14(11):6697-701. doi: 10.7314/apjcp.2013.14.11.6697.
To explore the inhibiting effect and mechanism of Endostar injection concomitant with cryoablation on lung adenocarcinoma A549 xenografts in nude mice.
A total of 24 nude mice with subcutaneous xenografts of the A549 cell line were established and divided into 4 groups when the maximal diameters of tumors became 1 cm: control group, Endostar group, cryoablation group and combination group (Endostar concomitant with cryoablation). The nude mice were sacrificed after 21-days treatment, tumour tissues were removed to measure their volume, in situ test of TdT-mediated dUTP nick end labeling (TUNEL) was adopted to determine the cellular apoptosis around freezing injury zones, and immunohistochemical SP test was applied for the detection of micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression levels.
At 21-days after treatment, the growth velocities of control group, Endostar group, cryoablation group and combination group were 236.7 ± 51.2%, 220.0 ± 30.6%, 159.5 ± 29.3% and 103.3 ± 25.5% (P<0.01), while cellular apoptosis rates of tumors were 21.7 ± 2.34%, (22.17 ± 1.47)%, 38.3 ± 1.37% and 49.2 ± 1.72%, (P<0.01), respectively, according to the immunohistochemical test. MVD and VEGF expression levels in the combination group were both lower than in other groups (P<0.01), also being positively related (r=0.925, P<0.01).
Endostar can significantly improve the inhibitory effects of cryoablation on xenografts of lung adenocarcinoma A549, and the mechanism is probably associated with its function as an inhibitor of tumour neo-angiogenesis through down-regulating VEGF expression.
探讨恩度注射液联合冷冻消融对裸鼠肺腺癌A549移植瘤的抑制作用及机制。
建立24只皮下接种A549细胞系的裸鼠模型，待肿瘤最大直径达1 cm时分为4组：对照组、恩度组、冷冻消融组和联合组（恩度联合冷冻消融）。治疗21天后处死裸鼠，取出肿瘤组织测量体积，采用原位末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测冷冻损伤区周围细胞凋亡情况，应用免疫组织化学SP法检测微血管密度（MVD）及血管内皮生长因子（VEGF）表达水平。
治疗21天后，对照组、恩度组、冷冻消融组和联合组肿瘤生长速度分别为236.7±51.2%、220.0±30.6%、159.5±29.3%和103.3±25.5%（P<0.01）；免疫组化检测肿瘤细胞凋亡率分别为21.7±2.34%、（22.17±1.47）%、38.3±1.37%和49.2±1.72%（P<0.01）。联合组MVD及VEGF表达水平均低于其他组（P<0.01），且二者呈正相关（r=0.925，P<0.01）。
恩度可显著增强冷冻消融对肺腺癌A549移植瘤的抑制作用，其机制可能与下调VEGF表达、抑制肿瘤新生血管生成有关。
