Madrigal-Bujaidar Eduardo, Martino Roaro Laura, Garcia-Aguirre Karol, Garcia-Medina Sandra, Alvarez-Gonzalez Isela
Laboratorio de Genetica, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico E-mail :
Asian Pac J Cancer Prev. 2013;14(11):6851-6. doi: 10.7314/apjcp.2013.14.11.6851.
In the present report we determined the protective capacity of grapefruit juice (GJ) against molecular and cellular damage in azoxymethane (AOM) treated mice. Animals were daily administered GJ orally (0.8, 4.1, and 8.2 μl/g) for seven weeks, as well as intraperitoneally (ip) injected with AOM twice (weeks 2 and 3 of the assay). Control groups administered with water, with the high dose of GJ, and with AOM injected in weeks 2 and 3 were also included. The results showed a significant, dose-dependent protection of GJ on the number of colon aberrant crypts (AC) induced by AOM. The highest inhibitory effect was reached with the highest tested dose of GJ, decreasing ACF by 51% and 43% at weeks 4 and 7 of the assay. Regarding protein and lipid oxidation we also found a dose-dependent decrease caused with GJ in comparison with the increased levels produced by AOM. Therefore, our results established chemopreventive potential for GJ, and suggested effects related to its antioxidant capacity. Finally, we found that the tested agents induced neither micronuclei increase nor alteration in bone marrow cytotoxicity.
在本报告中，我们测定了葡萄柚汁（GJ）对用偶氮甲烷（AOM）处理的小鼠分子和细胞损伤的保护能力。动物连续七周每天口服GJ（0.8、4.1和8.2微升/克），并在实验的第2周和第3周两次腹腔注射AOM。还包括给予水、高剂量GJ以及在第2周和第3周注射AOM的对照组。结果显示，GJ对AOM诱导的结肠异常隐窝（AC）数量具有显著的剂量依赖性保护作用。在测试的最高剂量GJ下达到了最高抑制效果，在实验的第4周和第7周，ACF分别降低了51%和43%。关于蛋白质和脂质氧化，我们还发现与AOM产生的增加水平相比，GJ导致了剂量依赖性降低。因此，我们的结果确定了GJ的化学预防潜力，并表明其与抗氧化能力有关。最后，我们发现测试药物既未诱导微核增加，也未改变骨髓细胞毒性。
