Zackrisson G, Lagergård T, Lönnroth I
Acta Pathol Microbiol Immunol Scand C. 1986 Dec;94(6):227-31. doi: 10.1111/j.1699-0463.1986.tb02116.x.
A microplate enzyme-linked-immunosorbent assay (ELISA) for detection of antibodies against pertussis toxin, using fetuin as a spacer-layer between the solid phase and pertussis toxin, was compared to a method using pertussis toxin alone as antigen. Antibodies of IgG, IgM and IgA classes were studied in paired human sera. Raised antibody levels were demonstrated for ten children suffering from whooping cough. Antibody levels in sera from 20 healthy 4-year-old children showed high correlation to earlier experienced pertussis. No significant antibody titer changes against pertussis toxin were seen in children with adenovirus infection. The investigation shows that precoating with fetuin improves the sensitivity of the method as much as 5-30 times and makes the method useful in serological diagnosis of whooping cough.
一种用于检测抗百日咳毒素抗体的微孔板酶联免疫吸附测定（ELISA），使用胎球蛋白作为固相和百日咳毒素之间的间隔层，与一种仅使用百日咳毒素作为抗原的方法进行了比较。在配对的人血清中研究了IgG、IgM和IgA类抗体。在10名患百日咳的儿童中证实抗体水平升高。20名健康4岁儿童血清中的抗体水平与早期患百日咳的经历高度相关。腺病毒感染儿童中未观察到针对百日咳毒素的抗体滴度有显著变化。研究表明，用胎球蛋白预包被可使该方法的灵敏度提高5至30倍，并使该方法可用于百日咳的血清学诊断。
