Zackrisson G, Lagergård T, Trollfors B, Krantz I
Department of Clinical Bacteriology, University of Göteborg, Sweden.
J Clin Microbiol. 1990 Jul;28(7):1502-5. doi: 10.1128/jcm.28.7.1502-1505.1990.
Immunoglobulin A (IgA) antibodies against pertussis toxin (PT) and filamentous hemagglutinin (FHA) in 181 saliva samples obtained during various stages of pertussis from 112 patients were determined. Saliva samples obtained within 5 days after the onset of symptoms did not have detectable IgA antibodies against either of the two antigens. Of the samples obtained between 6 and 50 days after the onset of symptoms, 72% had antibodies against FHA but only 40% had antibodies against PT. With few exceptions, saliva samples obtained more than 50 days after the onset of symptoms contained antibodies against both antigens. In the 59 patients from whom paired saliva samples were obtained at intervals of 2 to 5 weeks, a significant increase in the geometric mean FHA antibody titers but not PT antibody titers occurred. However, increases that were fourfold or greater were observed against FHA in only 19 patients and against PT in 14 patients. Thus, IgA antibodies against FHA and PT in saliva develop during pertussis, and the importance of secretory IgA antibodies for protection against infection and disease should be investigated. Determination of these antibodies in paired saliva samples is, however, of little value for the laboratory diagnosis of pertussis.
对112例患者在百日咳不同阶段采集的181份唾液样本中抗百日咳毒素（PT）和丝状血凝素（FHA）的免疫球蛋白A（IgA）抗体进行了测定。症状出现后5天内采集的唾液样本中未检测到针对这两种抗原中任何一种的IgA抗体。在症状出现后6至50天采集的样本中，72%有抗FHA抗体，但只有40%有抗PT抗体。除少数例外，症状出现后50天以上采集的唾液样本含有针对两种抗原的抗体。在59例每隔2至5周采集配对唾液样本的患者中，FHA抗体几何平均滴度显著升高，但PT抗体滴度未升高。然而，仅在19例患者中观察到FHA抗体升高四倍或更高，在14例患者中观察到PT抗体升高四倍或更高。因此，百日咳期间唾液中会产生抗FHA和PT的IgA抗体，分泌型IgA抗体对预防感染和疾病的重要性值得研究。然而，在配对唾液样本中测定这些抗体对百日咳的实验室诊断价值不大。
