Sun Li-mei, Li Hui, Tan Xiao-hua, Fang Ling, Deng Ai-ping, Mo Yan-ling, He Jian-feng, Ke Chang-wen, Lin Jin-yan
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China. Email:
Zhonghua Liu Xing Bing Xue Za Zhi. 2013 Oct;34(10):989-92.
To analyze the epidemiological and etiological characteristics of diarrheal disease among children under 5 years of age in Guangdong province, in 2012.
64 hospitals in 21 cities were chosen as the diarrheal syndromic surveillance sites, of which 14 hospitals were selected to carry out etiological surveillance among children under 5 years of age, including isolation and culture of both Vibrio cholera and Shigella as well as nucleic acid detection of rotavirus and norovirus by PCR. Descriptive method was used to analyze data from syndromic and etiological surveillance programs on diarrheal, from 1932 parents of the children.
In 2012, the outpatient attendance rate on diarrheal among children under 5 years was 0.8%. The proportion of diarrheal in children under 5-year-olds was 63.5%, among the total number of diarrheal outpatients at the outpatient clinics under surveillance program. The morbidity of infectious diarrhea was 1454.5/10 million in children under 5 years of age. A total number of 1932 specimens were collected from children under 5 years of age, in the outpatient department. Among these specimens,Vibrio cholera appeared all negative but one was Shigella positive and proved to be Sh. sonnei. The positive rates of rotavirus and norovirus were 14.1% (273/1932)and 16.9% (326/1932). Both rotavirus and norovirus were found in 24 specimens, with a positive rate as 1.2% . 112 specimens were successfully gene sequenced for rotavirus, of which 33.9% as G1[P8] genotype, 25.9% as G9[P8], 12.5% as G2[P4] and 9.8% as G3[P8] respectively. 90 specimens were successfully gene-sequenced for norovirus, of which 76.7% as G II.4 genotype. Genetic subtypes of G II. 4/2006b, accounted for 50.0% and could be detected around the year except for June and December. New G II. 4/Sydney Strain_2012 was first detected in August and became the predominant in December. In addition, 5 specimens belonged to G I genotype with other 16 subtypes of G II.
Results from our study proved that children under 5 years of age belonged to high-risk group for diarrheal disease in Guangdong province. Rotavirus and norovirus were both diverse in terms of genome.
分析2012年广东省5岁以下儿童腹泻病的流行病学及病因学特征。
选取21个城市的64家医院作为腹泻综合征监测点，其中14家医院对5岁以下儿童开展病因学监测，包括霍乱弧菌和志贺菌的分离培养以及采用聚合酶链反应（PCR）法进行轮状病毒和诺如病毒核酸检测。采用描述性方法分析1932名儿童家长提供的腹泻综合征及病因学监测数据。
2012年，5岁以下儿童腹泻门诊就诊率为0.8%。在监测门诊腹泻门诊患者总数中，5岁以下儿童腹泻占比为63.5%。5岁以下儿童感染性腹泻发病率为1454.5/1000万。门诊共采集1932份5岁以下儿童标本。其中，霍乱弧菌检测结果均为阴性，但有1份志贺菌阳性，经鉴定为宋内志贺菌。轮状病毒和诺如病毒阳性率分别为14.1%（273/1932）和16.9%（326/1932）。24份标本同时检出轮状病毒和诺如病毒，阳性率为1.2%。对112份轮状病毒标本成功进行基因测序，其中G1[P8]基因型占33.9%，G9[P8]占25.9%，G2[P4]占12.5%，G3[P8]占9.8%。对90份诺如病毒标本成功进行基因测序，其中76.7%为G II.4基因型。G II. 4/2006b基因亚型占50.0%，除6月和12月外全年均可检出。新型G II. 4/悉尼毒株_2012于8月首次检出，12月成为优势毒株。此外，5份标本属于G I基因型，另有16种G II基因亚型。
研究结果表明，5岁以下儿童是广东省腹泻病的高危人群。轮状病毒和诺如病毒在基因组方面均具有多样性。
