National Centre for Disease Control, Tripoli, Libya.
J Med Virol. 2011 Oct;83(10):1849-56. doi: 10.1002/jmv.22141.
Rotaviruses and noroviruses are leading viral causes of diarrhoea in children. A cross-sectional study was undertaken among children aged <5 years with acute gastroenteritis at Al-Jala Children's Hospital, Tripoli, Libya, from October 2007 to September 2008. Of 1,090 fecal samples collected, 260 from inpatients and 830 from outpatients, all inpatients and approximately a third of outpatients, selected systematically, were investigated for rotavirus and norovirus infection by ELISA and real-time RT-PCR, respectively. Of 520 fecal samples examined (inpatients = 260, outpatients = 260), 164 (31.5%) had rotavirus and 91 (17.5%) had norovirus detected. Rotavirus was identified more often among inpatients than outpatients (35.8% vs. 27.3% respectively, P = 0.038). Norovirus was detected more commonly among outpatients than inpatients (21.2% vs. 13.8% respectively, P = 0.028). The peak incidence of infection with both viruses was among children aged between 6 and 11 months. The number of rotavirus cases was highest between November and June with a peak detection rate of 50% in January. Norovirus occurred most commonly from May through August with a peak detection rate of 47% in August. The most prevalent rotavirus genotypes were P[8], G9 (n = 116, 65.9%), followed by P[8],G1 (n = 49, 27.8%); a single P[9], G3 strain was detected. There were seven distinct electropherotypes among the G9 strains and all belonged to VP7 Lineage III. Among 91 noroviruses identified, 90 were genogroup II. Of 26 genogroup II noroviruses examined, all were genotype GII.4. Rotaviruses and noroviruses are both important causes of gastrointestinal infection among young children in Libya.
轮状病毒和诺如病毒是导致儿童腹泻的主要病毒病原体。2007 年 10 月至 2008 年 9 月，在利比亚的黎波里的 Al-Jala 儿童医院，对年龄小于 5 岁的患有急性肠胃炎的儿童进行了一项横断面研究。在采集的 1090 份粪便样本中，住院患者采集了 260 份，门诊患者采集了 830 份，所有住院患者和大约三分之一的门诊患者都进行了系统选择，并分别通过 ELISA 和实时 RT-PCR 检测轮状病毒和诺如病毒感染。在 520 份粪便样本中（住院患者 260 份，门诊患者 260 份），检测到 164 份（31.5%）轮状病毒和 91 份（17.5%）诺如病毒。住院患者中轮状病毒的检出率高于门诊患者（分别为 35.8%和 27.3%，P=0.038）。门诊患者中诺如病毒的检出率高于住院患者（分别为 21.2%和 13.8%，P=0.028）。两种病毒的感染高峰期均在 6 至 11 个月龄的儿童中。轮状病毒病例数量最高，在 11 月至 6 月之间，1 月的检出率最高为 50%。诺如病毒最常发生在 5 月至 8 月之间，8 月的检出率最高为 47%。最常见的轮状病毒基因型是 P[8]、G9（n=116，65.9%），其次是 P[8]、G1（n=49，27.8%）；检测到一种单一的 P[9]、G3 株。在 91 株诺如病毒中，90 株为基因 II 组。在 26 株基因 II 组诺如病毒中，全部为 GII.4 基因型。轮状病毒和诺如病毒都是利比亚幼儿胃肠道感染的重要病原体。
