Zhao Haiyan, Wang Yalan, Ma Zhitao, Wang Yongqiang, Feng Wen-hai
State Key Laboratory of Agrobiotechnology, Key Laboratory of Soil Microbiology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
J Vet Sci. 2014;15(2):199-208. doi: 10.4142/jvs.2014.15.2.199. Epub 2013 Dec 27.
Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly, the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been used as an expression vehicle to elicit immune responses to foreign antigens. In this report, recombinant Kluyveromyces lactis expressing GP5 of HP-PRRSV (Yeast-GP5) was generated and immune responses to this construct were analyzed in mice. Intestinal mucosal PRRSV-specific sIgA antibody and higher levels of IFN-γ in spleen CD4(+) and CD8(+) T cells were induced by oral administration of Yeast-GP5. Additionally, Yeast-GP5 administered subcutaneously evoked vigorous cell-mediated immunity, and PRRSV-specific lymphocyte proliferation and IFN-γ secretion were detected in the splenocytes of mice. These results suggest that Yeast-GP5 has the potential for use as a vaccine for PRRSV in the future.
目前，灭活病毒疫苗和改良活猪繁殖与呼吸综合征病毒（PRRSV）疫苗被用于控制猪繁殖与呼吸综合征。然而，这两种疫苗都有其固有的缺点；因此，迫切需要开发新型PRRSV疫苗。先前的研究表明，酵母具有佐剂活性，并且已被用作表达载体以引发针对外源抗原的免疫反应。在本报告中，构建了表达高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）的GP5蛋白的重组乳酸克鲁维酵母（酵母-GP5），并在小鼠中分析了对该构建体的免疫反应。口服酵母-GP5可诱导肠道黏膜PRRSV特异性分泌型免疫球蛋白A（sIgA）抗体以及脾脏CD4(+)和CD8(+) T细胞中更高水平的干扰素-γ（IFN-γ）。此外，皮下注射酵母-GP5可引发强烈的细胞介导免疫反应，并且在小鼠脾细胞中检测到PRRSV特异性淋巴细胞增殖和IFN-γ分泌。这些结果表明，酵母-GP5未来有潜力用作PRRSV疫苗。
