Lightman S L, Caspers-Velu L E, Hirose S, Nussenblatt R B, Palestine A G
Arch Ophthalmol. 1987 Jun;105(6):844-8. doi: 10.1001/archopht.1987.01060060130048.
Sequential fluorescein angiography, using fluorescein-labeled dextran molecules of several different sizes, was carried out in monkeys with intraocular inflammation induced with interphotoreceptor retinoid-binding protein. The vascular leakage seen with the dextrans was compared with that seen with standard fluorescein sodium angiography. The angiograms demonstrated that different-sized leaks appear in the retinal vessels in adjacent areas during the course of the inflammation. Most retinal vessels leaked only fluorescein sodium and no dextran of any size, suggesting that it is the unbound fluorescein that leaks out of these vessels and not fluorescein bound to plasma albumin. It was not possible to tell by clinical examination which areas would leak the larger-molecular weight tracers. Ultrastructural studies of the veins leaking the dextrans revealed areas of abnormal endothelial tight junctions, whereas the tight junctions were normal in areas where leakage occurred with fluorescein alone.
使用几种不同大小的荧光素标记葡聚糖分子进行了连续荧光素血管造影，对因光感受器间类视黄醇结合蛋白诱导而发生眼内炎症的猴子进行了此项检查。将葡聚糖所显示的血管渗漏与标准荧光素钠血管造影所显示的渗漏进行了比较。血管造影显示，在炎症过程中，相邻区域的视网膜血管会出现不同大小的渗漏。大多数视网膜血管仅渗漏荧光素钠，不渗漏任何大小的葡聚糖，这表明从这些血管中渗漏出来的是未结合的荧光素，而非与血浆白蛋白结合的荧光素。通过临床检查无法判断哪些区域会渗漏较大分子量的示踪剂。对渗漏葡聚糖的静脉进行的超微结构研究显示，内皮紧密连接存在异常区域，而仅渗漏荧光素的区域，其紧密连接则是正常的。
