College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P.R. China.
Department of Radiotherapy, NanFang Hospital, Southern Medical University, Guangzhou 510515, P.R. China.
Int J Oncol. 2014 Mar;44(3):858-64. doi: 10.3892/ijo.2013.2237. Epub 2013 Dec 30.
Long non-coding RNAs (lncRNAs) are aberrantly expressed and have important functions in pathological processes. The present study investigated the lncRNA profiles and the effects of curcumin (Cur) on the radiosensitivity of nasopharyngeal carcinoma (NPC) cells. The lncRNA and mRNA profiles of each cell group were described by microarray analysis. Numerous differentially expressed genes were observed by microarrays in three cell groups. Cur significantly reversed the IR-induced lncRNA and mRNA expression signatures, shown by clustering analysis. Moreover, 116 of these IR-induced and Cur-reversed differentially expressed lncRNAs were obtained. Six lncRNAs (AF086415, AK095147, RP1-179N16.3, MUDENG, AK056098 and AK294004) were confirmed by qPCR. Furthermore, functional studies showed that lncRNA AK294004 exhibited a negative effect on cyclin D1 (CCND1), indicating that CCND1 might be a direct target of AK294004. IR-induced differentially expressed lncRNAs were reversed during Cur-enhanced radiosensitization in NPC cells, suggesting that lncRNAs have important functions in IR-induced radioresistance. Thus, Cur could serve as a good radiosensitizer.
长链非编码 RNA（lncRNA）在病理过程中表达异常，具有重要功能。本研究探讨了 lncRNA 谱和姜黄素（Cur）对鼻咽癌（NPC）细胞放射敏感性的影响。通过微阵列分析描述了每组细胞的 lncRNA 和 mRNA 谱。三组细胞的微阵列观察到许多差异表达的基因。聚类分析显示，Cur 显著逆转了 IR 诱导的 lncRNA 和 mRNA 表达特征。此外，还获得了 116 个 IR 诱导和 Cur 逆转的差异表达 lncRNA。qPCR 验证了其中 6 个 lncRNA（AF086415、AK095147、RP1-179N16.3、MUDENG、AK056098 和 AK294004）。此外，功能研究表明 lncRNA AK294004 对细胞周期蛋白 D1（CCND1）表现出负效应，表明 CCND1 可能是 AK294004 的直接靶标。在 NPC 细胞中，Cur 增强放射增敏作用时，IR 诱导的差异表达 lncRNA 被逆转，这表明 lncRNA 在 IR 诱导的放射抵抗中具有重要功能。因此，Cur 可以作为一种良好的放射增敏剂。
