人结肠癌中 cryptic overexpression 的临床病理和生物学意义。

Clinicopathological and biological significance of cripto overexpression in human colon cancer.

机构信息

Peng-Cheng Jiang, Hao-Liang Zhao, Department of General Surgery, Affiliated First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.

出版信息

World J Gastroenterol. 2013 Dec 14;19(46):8630-7. doi: 10.3748/wjg.v19.i46.8630.

DOI:10.3748/wjg.v19.i46.8630

PMID:24379580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3870508/

Abstract

AIM

To assess the clinicopathological and biological significance of cripto in human colorectal cancer.

METHODS

Real-time reverse-transcription polymerase chain reaction (PCR) was used to examine cripto mRNA levels in primary colon cancer and normal colon tissues as well as normal and metastatic lymph nodes from colon cancers. Human colon cancer LS-174T cells were transfected with cripto small interfering RNA (siRNA), and mRNA and protein levels were evaluated using real-time PCR and western blot analysis, respectively. The growth of cancer cells was evaluated using the MTT assay and colony formation in soft agar. Invasion was examined using a Transwell assay, and the expressions of matrix metalloproteinase (MMP)-7 and MMP-9 were determined using western blot assay.

RESULTS

Cripto was significantly overexpressed in primary colon cancer and metastatic lymph nodes. Silencing cripto gene expression with cripto siRNA resulted in a significant decrease in colony formation in soft agar in the colon cancer cell line LS-174T. Cripto siRNA treatment decreased the migration and invasion capabilities of the colon cancer cell line LS-174T in vitro. Furthermore, cripto siRNA treatment inhibited the expression of matrix MMP-7 and MMP-9.

CONCLUSION

The results provide evidence that cripto siRNA could be an effective approach for the inhibition of cancer cell invasion and migration and thus has potential for use in devising novel preventive and therapeutic strategies for colon cancer metastasis.

摘要

目的

评估 crypt 在人结直肠癌中的临床病理和生物学意义。

方法

采用实时逆转录聚合酶链反应（PCR）检测原发结直肠癌及正常结肠组织、结直肠癌正常及转移淋巴结中 crypto mRNA 水平。用人结直肠癌细胞 LS-174T 转染 crypto 小干扰 RNA（siRNA），分别采用实时 PCR 和 Western blot 分析评估 mRNA 和蛋白水平。采用 MTT 试验评估癌细胞生长，采用软琼脂集落形成试验评估集落形成，采用 Transwell 试验检测侵袭，采用 Western blot 试验检测基质金属蛋白酶（MMP）-7 和 MMP-9 的表达。

结果

crypt 在原发结直肠癌和转移淋巴结中明显过表达。用 crypto siRNA 沉默 crypto 基因表达可显著降低结直肠癌细胞系 LS-174T 软琼脂中的集落形成。crypto siRNA 处理可降低结直肠癌细胞系 LS-174T 的体外迁移和侵袭能力。此外，crypto siRNA 处理可抑制基质 MMP-7 和 MMP-9 的表达。