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匙羹藤的提取优化及其生物碱巴马汀的抗癌活性评估。

Extraction optimization of Tinospora cordifolia and assessment of the anticancer activity of its alkaloid palmatine.

作者信息

Ali Huma, Dixit Savita

机构信息

Department of Chemistry, MANIT, Bhopal, Madhya Pardesh, India.

出版信息

ScientificWorldJournal. 2013 Nov 28;2013:376216. doi: 10.1155/2013/376216. eCollection 2013.

DOI:10.1155/2013/376216
PMID:24379740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3863568/
Abstract

OBJECTIVE

To optimize the conditions for the extraction of alkaloid palmatine from Tinospora cordifolia by using response surface methodology (RSM) and study its anticancerous property against 7,12-dimethylbenz(a)anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice.

METHODS

The effect of three independent variables, namely, extraction temperature, time, and cycles was investigated by using central composite design. A single topical application of DMBA (100 μg/100 μL of acetone), followed 2 weeks later by repeated application of croton oil (1% in acetone three times a week) for 16 weeks, exhibited 100 percent tumor incidence (Group 2).

RESULTS

The highest yield of alkaloid from Tinospora cordifolia could be achieved at 16 hours of extraction time under 40°C with 4 extraction cycles. Alkaloid administration significantly decreases tumor size, number, and the activity of serum enzyme when compared with the control (Group 2). In addition, depleted levels of reduced glutathione (GSH), superoxide dismutase (SOD), and catalase and increased DNA damage were restored in palmatine treated groups.

CONCLUSION

The data of the present study clearly indicate the anticancer potential of palmatine alkaloid in DMBA induced skin cancer model in mice.

摘要

目的

采用响应面法（RSM）优化从毛青藤中提取生物碱巴马汀的条件，并研究其对7,12-二甲基苯并(a)蒽（DMBA）诱导的瑞士白化小鼠皮肤癌的抗癌特性。

方法

采用中心复合设计研究提取温度、时间和次数这三个自变量的影响。单次局部涂抹DMBA（100μg/100μL丙酮），2周后每周3次重复涂抹巴豆油（1%丙酮溶液），持续16周，肿瘤发生率达100%（第2组）。

结果

在40℃下提取16小时、4次提取循环时，从毛青藤中提取的生物碱产量最高。与对照组（第2组）相比，生物碱给药显著减小肿瘤大小、减少肿瘤数量并降低血清酶活性。此外，巴马汀治疗组中降低的还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶水平以及增加的DNA损伤得到恢复。

结论

本研究数据清楚地表明了巴马汀生物碱在DMBA诱导的小鼠皮肤癌模型中的抗癌潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/3c8696081bef/TSWJ2013-376216.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/aa4dea0be75c/TSWJ2013-376216.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/32c35d904eae/TSWJ2013-376216.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/ca3bdf221b24/TSWJ2013-376216.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/a256ffde1e98/TSWJ2013-376216.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/3c8696081bef/TSWJ2013-376216.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/aa4dea0be75c/TSWJ2013-376216.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/32c35d904eae/TSWJ2013-376216.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/ca3bdf221b24/TSWJ2013-376216.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/a256ffde1e98/TSWJ2013-376216.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf2e/3863568/3c8696081bef/TSWJ2013-376216.005.jpg

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