Ito Caren Naomi Aguero, Santos Procopio Elisangela Dos, Balsalobre Natália de Matos, Machado Lucas Luiz, Silva-Filho Saulo Euclides, Pedroso Taíse Fonseca, Lourenço Caroline Caramano de, Oliveira Rodrigo Juliano, Arena Arielle Cristina, Salvador Marcos José, Kassuya Cândida Aparecida Leite
Health Sciences College, Federal University of Grande Dourados (UFGD), Dourados 79804-970, MS, Brazil.
Pharmaceutical Sciences, Food and Nutrition College, Federal University of Mato Grosso do Sul (UFMS), Campo Grande 79070-900, MS, Brazil.
Pharmaceuticals (Basel). 2024 Oct 5;17(10):1331. doi: 10.3390/ph17101331.
: is used in folk medicine to treat pain and arthritis. Palmatine is an alkaloid isolated from several plants, including leaves. The aim of the present study was to investigate the analgesic, anti-arthritic, and anti-inflammatory potential of the methanolic extract of (EMAS) and palmatine. : The chemical profile of EMAS was evaluated by ultra high-performance liquid chromatography with electrospray ionization coupled to mass spectrometry (UHPLC-ESI/MS). EMAS and palmatine were evaluated in carrageenan-induced pleurisy, zymosan-induced joint inflammation, formalin-induced nociception, and tumor necrosis factor (TNF)-induced mechanical hyperalgesia in experimental models in mice. A cytotoxicity test of EMAS and palmatine was performed using a methylthiazolidiphenyl-tetrazolium (MTT) bromide assay. : The analysis of the chemical profile of the extract showed the presence of palmatine, liriodenine, and anonaine. Oral administration of EMAS and palmatine significantly reduced leukocyte migration and oxide nitric production in the carrageenan-induced pleurisy model. EMAS and palmatine reduced mechanical hyperalgesia, leukocyte migration, and edema formation in the joint inflammation induced by zymosan. In the formalin test, palmatine was effective against the second-phase nociceptive response, mechanical hyperalgesia, and cold allodynia. In addition, palmatine reduced mechanical hyperalgesia induced by TNF. EMAS and palmatine did not demonstrate cytotoxicity. : The present study showed that and palmatine are analgesic and anti-inflammatory agents, and that the anti-hyperalgesic properties of palmatine may involve the TNF pathway. Palmatine may be one of the compounds responsible for the anti-hyperalgesic and/or anti-arthritic properties of this medicinal plant.
在民间医学中用于治疗疼痛和关节炎。巴马汀是从包括[植物名称]叶在内的几种植物中分离出的一种生物碱。本研究的目的是研究[植物名称]甲醇提取物（EMAS）和巴马汀的镇痛、抗关节炎和抗炎潜力。方法：通过超高效液相色谱-电喷雾电离-质谱联用（UHPLC-ESI/MS）对EMAS的化学特征进行评估。在小鼠实验模型中，对EMAS和巴马汀进行角叉菜胶诱导的胸膜炎、酵母聚糖诱导的关节炎症、福尔马林诱导的伤害感受以及肿瘤坏死因子（TNF）诱导的机械性痛觉过敏实验。使用甲基噻唑基二苯基四氮唑溴盐（MTT）法对EMAS和巴马汀进行细胞毒性测试。结果：提取物的化学特征分析显示存在巴马汀、鹅掌楸碱和去甲乌药碱。口服EMAS和巴马汀可显著减少角叉菜胶诱导的胸膜炎模型中的白细胞迁移和一氧化氮生成。EMAS和巴马汀可减轻酵母聚糖诱导的关节炎症中的机械性痛觉过敏、白细胞迁移和水肿形成。在福尔马林试验中，巴马汀对第二阶段伤害性反应、机械性痛觉过敏和冷觉异常有效。此外，巴马汀可减轻TNF诱导的机械性痛觉过敏。EMAS和巴马汀未显示细胞毒性。结论：本研究表明[植物名称]和巴马汀具有镇痛和抗炎作用，且巴马汀的抗痛觉过敏特性可能涉及TNF途径。巴马汀可能是该药用植物具有抗痛觉过敏和/或抗关节炎特性的化合物之一。
