Hassanzadeh Taghi, Maleki Monireh, Saidijam Massoud, Paoli Max
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ; Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
J Res Med Sci. 2013 Aug;18(8):668-73.
Metabolic syndrome (MetS) is a serious public health problem. It is an important risk factor of cardiovascular disease in developed countries. Adipose tissue considered as an organ that releases a variety of molecules referred to adipocytokines such as leptin. Polymorphism of their related genes may play an important role in development of MetS. The aim of this study was to determine the association of leptin gene-2548G/A (LEP-2548G/A) polymorphism with lipid profile in subjects with and without Mets.
In this case/control study a frequency of LEP-2548G/A single nucleotide polymorphism was determined between 200 patients (142 women and 58 men) and 200 controls (122 women and 78 men). Both groups were selected randomly from Hamadan city, Iran. Blood samples were collected then followed by routine biochemical analysis, DNA extraction and serum leptin measurements. Polymerase chain reaction-restriction fragment length polymorphism was applied to identify LEP-2548G/A genotypes. Statistical analyses were applied using SPSS software version 10. Continuous variables were presented as means± SD and compared by independent sample t-test. Variables without normal distribution compared through Mann-Whitney U test.
In both groups, a significant difference was observed between biochemical factors and leptin concentration. Serum leptin concentration was more in females than males. No statistical significant difference was detected in the frequency of LEP-2548G/A polymorphism between both MetS and healthy groups.
In summary, it is concluded that frequency of LEP G-2548A polymorphism in Metabolic syndrome (MetS) and healthy subjects was not significantly different and more research with large sample size is needed in this area.
代谢综合征（MetS）是一个严重的公共卫生问题。在发达国家，它是心血管疾病的一个重要危险因素。脂肪组织被视为一个能释放多种分子（即脂肪细胞因子，如瘦素）的器官。其相关基因的多态性可能在代谢综合征的发生发展中起重要作用。本研究的目的是确定瘦素基因-2548G/A（LEP-2548G/A）多态性与患有和未患有代谢综合征的受试者血脂水平之间的关联。
在这项病例对照研究中，测定了200例患者（142名女性和58名男性）和200名对照者（122名女性和78名男性）中LEP-2548G/A单核苷酸多态性的频率。两组均从伊朗哈马丹市随机选取。采集血样，随后进行常规生化分析、DNA提取和血清瘦素测量。应用聚合酶链反应-限制性片段长度多态性来鉴定LEP-2548G/A基因型。使用SPSS 10.0软件进行统计分析。连续变量以均数±标准差表示，并通过独立样本t检验进行比较。非正态分布的变量通过曼-惠特尼U检验进行比较。
两组在生化因素和瘦素浓度方面均观察到显著差异。女性血清瘦素浓度高于男性。代谢综合征组和健康组之间LEP-2548G/A多态性频率未检测到统计学显著差异。
总之，得出的结论是代谢综合征（MetS）患者和健康受试者中LEP G-2548A多态性频率无显著差异，该领域需要进行更多大样本量的研究。
