Ben Ali S, Kallel A, Ftouhi B, Sediri Y, Feki M, Slimane H, Jemaa R, Kaabachi N
Research Laboratory LAB-SM-01, Biochemistry Department, Rabta Hospital, Tunis, Tunisia.
Blood Press. 2008;17(5-6):278-83. doi: 10.1080/08037050802488960.
To examine the association of a common -2548G/A (rs7799039) promoter variant of the human leptin gene (LEP) with obesity or body mass index (BMI) and its associated phenotypes such as blood pressure variability and the prevalence of hypertension in a sample of the Tunisian population.
Two hundred and twenty-nine obese patients were screened and compared with 251 normal weight subjects. The -2548G/A LEP polymorphism was analysed by PCR-RFLP procedure.
No significant association was found between the -2548G/A polymorphism and obesity or BMI. However, in obese patients subjects with AA genotype had significantly higher systolic (p = 0.003) and diastolic (p = 0.002) blood pressure compared with those with GA or GG genotypes. Stratified analysis by gender revealed that male patients but not female homozygous for -2548A allele exhibited significantly increased systolic (p = 0.01) and diastolic (p<0.001) blood pressure than did carriers of -2548G allele. Multiple linear regression analysis revealed that AA genotype significantly affect systolic and diastolic blood pressure in obese men. Additionally, significant association between AA genotype and higher prevalence of hypertension was found in male patients (p = 0.03).
The present study showed that the -2548G/A LEP polymorphism is associated with blood pressure in obese male patients.
在突尼斯人群样本中，研究人类瘦素基因（LEP）常见的 -2548G/A（rs7799039）启动子变异与肥胖或体重指数（BMI）及其相关表型（如血压变异性和高血压患病率）之间的关联。
对229名肥胖患者进行筛查，并与251名正常体重受试者进行比较。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析 -2548G/A LEP多态性。
未发现 -2548G/A多态性与肥胖或BMI之间存在显著关联。然而，在肥胖患者中，与GA或GG基因型患者相比，AA基因型患者的收缩压（p = 0.003）和舒张压（p = 0.002）显著更高。按性别分层分析显示，-2548A等位基因纯合的男性患者而非女性患者，其收缩压（p = 0.01）和舒张压（p<0.001）比 -2548G等位基因携带者显著升高。多元线性回归分析显示，AA基因型显著影响肥胖男性的收缩压和舒张压。此外，在男性患者中发现AA基因型与高血压患病率较高之间存在显著关联（p = 0.03）。
本研究表明，-2548G/A LEP多态性与肥胖男性患者的血压相关。
