盐酸川芎嗪注射液对诱导性动静脉分流血栓形成模型的抗血栓作用。
Antithrombotic effect of ligustrazine hydrochloride injection on the model of induced arteriovenous shunt thrombosis.
作者信息
Wang Peng, Luo Chun-Hua, Wang Qun-Xing, Li Qian-Yuan, Li Peng, Yuan Xiao-Hui
机构信息
Department of Clinical Laboratory, The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Institute of Biomedicine, Jinan University, Guangzhou, China.
出版信息
J Res Med Sci. 2013 Aug;18(8):704-6.
BACKGROUND
The objective of this study is to optimize the effective dose of heparin and ligustrazine hydrochloride injection (LHI) for drug combination.
MATERIALS AND METHODS
The animal clinical study of LHI was performed by the rat's model of induced arteriovenous shunt thrombosis. Experimental animals were grouped into several groups and separately treated with both LHI (20, 40, 80 mg/kg, i.p.) and heparin (60, 55, and 50 U/kg; 5000 U/ml; Sigma, i.v). The study had used thrombus weight, protein concentration in thrombus homogenate, inhibition rate of thrombosis, and plasma anti-thrombin activity as indications.
RESULTS
The group combination (50, 80) got the result of 100% antithrombotic activity with 0 ± 0 mg of thrombus weight, 14 ± 3 μg/ml of protein concentration in thrombus homogenate and 1.5 ± 0.04 U/ml of plasma anti-thrombin activity. Its anti-thrombotic effect was much better than individual groups treated with LHI in a dose of 0 mg/kg and group of combination (0, 80) (P < 0.05) while antithrombotic effect of 55 and 60 U/kg heparin alone was only 37-58%. Therefore, the group of combination (50, 80) was optimal for 100% antithrombotic activity.
CONCLUSION
Optimal combined doses of LHI and heparin preventing blood coagulation were determined and the results were available. It may give some hint for the further clinical application on human.
背景
本研究的目的是优化肝素与盐酸川芎嗪注射液（LHI）联合用药的有效剂量。
材料与方法
采用大鼠动静脉分流血栓形成模型进行LHI的动物临床研究。将实验动物分成几组，分别用LHI（20、40、80mg/kg，腹腔注射）和肝素（60、55和50U/kg；5000U/ml；Sigma，静脉注射）进行治疗。本研究以血栓重量、血栓匀浆中的蛋白质浓度、血栓形成抑制率和血浆抗凝血酶活性作为指标。
结果
组合组（50，80）的抗血栓活性结果为100%，血栓重量为0±0mg，血栓匀浆中的蛋白质浓度为14±3μg/ml，血浆抗凝血酶活性为1.5±0.04U/ml。其抗血栓作用明显优于LHI剂量为0mg/kg的单独治疗组和组合组（0，80）（P<0.05），而单独使用55和60U/kg肝素的抗血栓作用仅为37-58%。因此，组合组（50，80）具有100%的最佳抗血栓活性。
结论
确定了LHI与肝素预防血液凝固的最佳联合剂量，结果可供参考。这可能为进一步在人体上的临床应用提供一些线索。
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