• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells.高通量测序揭示了诱导多能干细胞中印迹基因甲基化的破坏。
Cell Res. 2014 Mar;24(3):293-306. doi: 10.1038/cr.2013.173. Epub 2013 Dec 31.
2
Identification of the new gene Zrsr1 to associate with the pluripotency state in induced pluripotent stem cells (iPSCs) using high throughput sequencing technology.利用高通量测序技术鉴定与诱导多能干细胞（iPSC）多能性状态相关的新基因Zrsr1。
Genom Data. 2014 Apr 30;2:73-7. doi: 10.1016/j.gdata.2014.04.008. eCollection 2014 Dec.
3
Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1γ in reprogramming to pluripotency.蛋白质组学和基因组学方法揭示了 H3K9 甲基化和异染色质蛋白-1γ在重编程为多能性中的关键功能。
Nat Cell Biol. 2013 Jul;15(7):872-82. doi: 10.1038/ncb2768. Epub 2013 Jun 9.
4
High throughput sequencing identifies an imprinted gene, Grb10, associated with the pluripotency state in nuclear transfer embryonic stem cells.高通量测序鉴定出一个与核移植胚胎干细胞多能性状态相关的印记基因Grb10。
Oncotarget. 2017 Jul 18;8(29):47344-47355. doi: 10.18632/oncotarget.17185.
5
The Epigenetic Reprogramming Roadmap in Generation of iPSCs from Somatic Cells.体细胞生成 iPS 细胞中的表观遗传重编程路线图。
J Genet Genomics. 2015 Dec 20;42(12):661-70. doi: 10.1016/j.jgg.2015.10.001. Epub 2015 Oct 23.
6
Residual expression of reprogramming factors affects the transcriptional program and epigenetic signatures of induced pluripotent stem cells.重编程因子的残留表达会影响诱导多能干细胞的转录程序和表观遗传特征。
PLoS One. 2012;7(12):e51711. doi: 10.1371/journal.pone.0051711. Epub 2012 Dec 14.
7
Nuclear transcriptome profiling of induced pluripotent stem cells and embryonic stem cells identify non-coding loci resistant to reprogramming.诱导多能干细胞和胚胎干细胞的核转录组分析鉴定出对重编程有抗性的非编码基因座。
Cell Cycle. 2015;14(8):1148-55. doi: 10.4161/15384101.2014.988031.
8
X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs.X 染色体剂量对重编程为小鼠诱导多能干细胞过程中的 DNA 甲基化动力学的影响。
Stem Cell Reports. 2018 May 8;10(5):1537-1550. doi: 10.1016/j.stemcr.2018.03.019. Epub 2018 Apr 19.
9
Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs.造血分化结果与分子特征的综合分析揭示了造血祖细胞/造血干细胞诱导多能干细胞（HPC/HSC-iPSCs）中无偏倚的分化能力和微小的转录记忆。
Stem Cell Res Ther. 2017 Jan 23;8(1):13. doi: 10.1186/s13287-016-0466-1.
10
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.H3K9 甲基化是体细胞重编程为 iPS 细胞过程中的一个障碍。
Nat Genet. 2013 Jan;45(1):34-42. doi: 10.1038/ng.2491. Epub 2012 Dec 2.

引用本文的文献

1
Identification of Hub of the Hub-Genes From Different Individual Studies for Early Diagnosis, Prognosis, and Therapies of Breast Cancer.从不同个体研究中鉴定乳腺癌早期诊断、预后和治疗的核心基因枢纽
Bioinform Biol Insights. 2024 Sep 4;18:11779322241272386. doi: 10.1177/11779322241272386. eCollection 2024.
2
Promoting GSDME expression through DNA demethylation to increase chemosensitivity of breast cancer MCF-7 / Taxol cells.通过 DNA 去甲基化促进 GSDME 表达以增加乳腺癌 MCF-7/Taxol 细胞的化疗敏感性。
PLoS One. 2023 Mar 3;18(3):e0282244. doi: 10.1371/journal.pone.0282244. eCollection 2023.
3
BrewerIX enables allelic expression analysis of imprinted and X-linked genes from bulk and single-cell transcriptomes.BrewerIX 可从转录组的混合样本和单细胞中分析印迹基因和 X 连锁基因的等位基因表达。
Commun Biol. 2022 Feb 17;5(1):146. doi: 10.1038/s42003-022-03087-4.
4
Minor Splicing Factors and Are Essential for Early Embryo Development and 2-Cell-Like Conversion.少量剪接因子对于早期胚胎发育和 2 细胞样转化至关重要。
Int J Mol Sci. 2020 Jun 9;21(11):4115. doi: 10.3390/ijms21114115.
5
Role of Long Non-coding RNAs in Reprogramming to Induced Pluripotency.长非编码 RNA 在重编程为诱导多能性中的作用。
Genomics Proteomics Bioinformatics. 2020 Feb;18(1):16-25. doi: 10.1016/j.gpb.2019.06.003. Epub 2020 May 21.
6
Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs.从山中因子组合中去除Oct4可释放诱导多能干细胞的发育潜能。
Cell Stem Cell. 2019 Dec 5;25(6):737-753.e4. doi: 10.1016/j.stem.2019.10.002. Epub 2019 Nov 7.
7
Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.从成纤维细胞直接诱导出三胚层前植入期囊胚细胞类型。
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
8
Conserved regulation of RNA processing in somatic cell reprogramming.体细胞重编程中 RNA 处理的保守调控。
BMC Genomics. 2019 Jan 31;20(1):100. doi: 10.1186/s12864-019-5438-2.
9
Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning.沉默逆转录转座子衍生的印迹基因 RTL1 是哺乳动物克隆植入后失败的主要原因。
Proc Natl Acad Sci U S A. 2018 Nov 20;115(47):E11071-E11080. doi: 10.1073/pnas.1814514115. Epub 2018 Oct 31.
10
Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells.胚胎生殖细胞提取物可消除印记基因，提高诱导多能干细胞的效率。
Sci Rep. 2018 Jul 19;8(1):10955. doi: 10.1038/s41598-018-29339-0.

本文引用的文献

1
FGF signaling inhibition in ESCs drives rapid genome-wide demethylation to the epigenetic ground state of pluripotency.FGF 信号抑制可促使胚胎干细胞快速进行全基因组去甲基化，达到多能性的表观遗传基础状态。
Cell Stem Cell. 2013 Sep 5;13(3):351-9. doi: 10.1016/j.stem.2013.06.004. Epub 2013 Jul 11.
2
Stability of genomic imprinting in human induced pluripotent stem cells.人类诱导多能干细胞中基因组印记的稳定性。
BMC Genet. 2013 Apr 30;14:32. doi: 10.1186/1471-2156-14-32.
3
Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation.Zscan4 在重编程过程中促进基因组稳定性，并通过四倍体互补显著提高 iPS 细胞的质量。
Cell Res. 2013 Jan;23(1):92-106. doi: 10.1038/cr.2012.157. Epub 2012 Nov 13.
4
Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm.人类精子中重组活性和新突变率的全基因组单细胞分析。
Cell. 2012 Jul 20;150(2):402-12. doi: 10.1016/j.cell.2012.06.030.
5
The transcriptional and epigenomic foundations of ground state pluripotency.胚胎干细胞多能性的转录和表观遗传基础。
Cell. 2012 Apr 27;149(3):590-604. doi: 10.1016/j.cell.2012.03.026.
6
Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells.抗坏血酸可防止 Dlk1-Dio3 印迹丢失，并促进终末分化 B 细胞生成全 iPS 细胞小鼠。
Nat Genet. 2012 Mar 4;44(4):398-405, S1-2. doi: 10.1038/ng.1110.
7
Base-resolution analyses of sequence and parent-of-origin dependent DNA methylation in the mouse genome.基于分辨率的分析序列和母源依赖性的 DNA 甲基化在老鼠的基因组。
Cell. 2012 Feb 17;148(4):816-31. doi: 10.1016/j.cell.2011.12.035.
8
Reprogramming factor stoichiometry influences the epigenetic state and biological properties of induced pluripotent stem cells.重编程因子的比例会影响诱导多能干细胞的表观遗传状态和生物学特性。
Cell Stem Cell. 2011 Dec 2;9(6):588-98. doi: 10.1016/j.stem.2011.11.003.
9
ChIP-Seq: technical considerations for obtaining high-quality data.ChIP-Seq：获取高质量数据的技术考虑因素。
Nat Immunol. 2011 Sep 20;12(10):918-22. doi: 10.1038/ni.2117.
10
Identification of novel transcripts in annotated genomes using RNA-Seq.利用 RNA-Seq 鉴定注释基因组中的新型转录本。
Bioinformatics. 2011 Sep 1;27(17):2325-9. doi: 10.1093/bioinformatics/btr355. Epub 2011 Jun 21.

高通量测序揭示了诱导多能干细胞中印迹基因甲基化的破坏。

High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells.

作者信息

Chang Gang, Gao Shuai, Hou Xinfeng, Xu Zijian, Liu Yanfeng, Kang Lan, Tao Yu, Liu Wenqiang, Huang Bo, Kou Xiaochen, Chen Jiayu, An Lei, Miao Kai, Di Keqian, Wang Zhilong, Tan Kun, Cheng Tao, Cai Tao, Gao Shaorong, Tian Jianhui

机构信息

1] Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National Engineering Laboratory for Animal Breeding, College of Animal Sciences and Technology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China [2] National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China.

National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China.

出版信息

Cell Res. 2014 Mar;24(3):293-306. doi: 10.1038/cr.2013.173. Epub 2013 Dec 31.

DOI:10.1038/cr.2013.173
PMID:24381111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3945885/
Abstract

It remains controversial whether the abnormal epigenetic modifications accumulated in the induced pluripotent stem cells (iPSCs) can ultimately affect iPSC pluripotency. To probe this question, iPSC lines with the same genetic background and proviral integration sites were established, and the pluripotency state of each iPSC line was characterized using tetraploid (4N) complementation assay. Subsequently, gene expression and global epigenetic modifications of "4N-ON" and the corresponding "4N-OFF" iPSC lines were compared through deep sequencing analyses of mRNA expression, small RNA profile, histone modifications (H3K27me3, H3K4me3, and H3K4me2), and DNA methylation. We found that methylation of an imprinted gene, Zrsr1, was consistently disrupted in the iPSC lines with reduced pluripotency. Furthermore, the disrupted methylation could not be rescued by improving culture conditions or subcloning of iPSCs. Moreover, the relationship between hypomethylation of Zrsr1 and pluripotency state of iPSCs was further validated in independent iPSC lines derived from other reprogramming systems.

摘要

诱导多能干细胞（iPSC）中积累的异常表观遗传修饰是否最终会影响iPSC的多能性仍存在争议。为探究这个问题，建立了具有相同遗传背景和原病毒整合位点的iPSC系，并使用四倍体（4N）互补试验对每个iPSC系的多能性状态进行了表征。随后，通过对mRNA表达、小RNA谱、组蛋白修饰（H3K27me3、H3K4me3和H3K4me2）以及DNA甲基化的深度测序分析，比较了“4N-ON”和相应的“4N-OFF”iPSC系的基因表达和整体表观遗传修饰。我们发现，在多能性降低的iPSC系中，印记基因Zrsr1的甲基化始终受到破坏。此外，通过改善培养条件或iPSC的亚克隆无法挽救被破坏的甲基化。此外，在源自其他重编程系统的独立iPSC系中进一步验证了Zrsr1的低甲基化与iPSC多能性状态之间的关系。