Baijot B, Duchene M, Stephenne J
Dev Biol Stand. 1987;66:523-30.
A process for the production of Aujeszky vaccine on microcarrier cultures in large fermentors is described. The NLST cells are routinely produced in fermentors of 150 litres; cell density reaches 0.7-0.8 10(6)/ml. The authors describe the method used to obtain those results. The different parameters used in fermentors are discussed and especially pO2 regulation. A study of subculturing method is being carried out to reach a volume of 500 litres. The comparison between stationary culture and microcarrier cultures shows the advantages of such a technique for the viral antigen produced versus infective and the increase of the bulk capacity. This experience also shows that specific development is required for each cell line.
描述了一种在大型发酵罐的微载体培养物上生产奥耶斯基疫苗的方法。NLST细胞通常在150升的发酵罐中生产；细胞密度达到0.7 - 0.8×10⁶/ml。作者描述了用于获得这些结果的方法。讨论了发酵罐中使用的不同参数，特别是pO₂调节。正在进行传代培养方法的研究以达到500升的体积。静态培养和微载体培养之间的比较显示了这种技术相对于感染性产生的病毒抗原的优势以及批量生产能力的提高。该经验还表明每个细胞系都需要特定的开发。
