Caij A, De Smet A, Dubois N, Koenen F
National Institute for Veterinary Research, Brussels, Belgium.
Arch Virol. 1989;105(1-2):113-8. doi: 10.1007/BF01311121.
In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3 microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.
为了制备高滴度的猪霍乱病毒（HCV）制剂，确定了PK-15微载体细胞培养的最佳生长和胰蛋白酶消化条件。与在鲁氏瓶中进行的传统单层培养相比，用HCV感染PK-15 Cytodex 3微载体培养物可使病毒产量提高10倍以上。
