Becker Christine, Klaering Hans-Peter, Schreiner Monika, Kroh Lothar W, Krumbein Angelika
Leibniz Institute for Vegetable and Ornamental Crops Grossbeeren/Erfurt e.V., Theodor-Echtermeyer-Weg 1, 14979 Grossbeeren, Germany.
J Agric Food Chem. 2014 Jul 23;62(29):6911-7. doi: 10.1021/jf404782n. Epub 2014 Jan 9.
This study investigated the effect of low-level photosynthetic photon flux density (PPFD; 43-230 μmol m(-2) s(-1)) on the major phenolic compounds of red leaf lettuce in three growth stages, before, during, and after head formation, using HPLC-DAD-ESI-MS(2) and evaluating via multiple regression analysis. Generally, the light-related increase of flavonoid glycosides was structure and growth stage-dependent. In detail, an interaction was detected between plant age and PPFD regarding cyanidin-3-O-(6"-O-malonyl)-glucoside concentration: the increase was strongest before head formation. The relationship between PPFD and quercetin-3-O-(6"-O-malonyl)-glucoside concentration was linear, whereas the increase of quercetin-3-O-glucoside and -3-O-glucuronide concentrations abated with increasing PPFD. Independent of growth stage, the caffeic acid derivatives concentration was not related to PPFD. All major phenolic compounds decreased with plant age. These results show the differential regulation of cyanidin, quercetin, and caffeic acid derivatives in lettuce, although closely connected biosynthetically, and emphasize the importance of ontogeny in the study of plant physiology.
本研究利用高效液相色谱-二极管阵列-电喷雾电离串联质谱（HPLC-DAD-ESI-MS(2)），通过多元回归分析，研究了低水平光合光子通量密度（PPFD；43 - 230 μmol m(-2) s(-1)）对红叶生菜在三个生长阶段（结球前、结球期和结球后）主要酚类化合物的影响。总体而言，黄酮苷类与光相关的增加取决于结构和生长阶段。具体来说，检测到植物年龄与PPFD在矢车菊素-3-O-(6"-O-丙二酰基)-葡萄糖苷浓度方面存在相互作用：在结球前增加最为显著。PPFD与槲皮素-3-O-(6"-O-丙二酰基)-葡萄糖苷浓度之间呈线性关系，而槲皮素-3-O-葡萄糖苷和-3-O-葡萄糖醛酸苷浓度的增加随着PPFD的升高而减弱。与生长阶段无关，咖啡酸衍生物浓度与PPFD无关。所有主要酚类化合物均随植物年龄增长而降低。这些结果表明，尽管花青素、槲皮素和咖啡酸衍生物在生物合成上紧密相连，但它们在生菜中的调控存在差异，并强调了个体发育在植物生理学研究中的重要性。
