Nishioka K, Katayama I, Kobayashi Y
J Invest Dermatol. 1987 Jun;88(6):694-8. doi: 10.1111/1523-1747.ep12470371.
Immune response-associated (Ia) antigens are important as molecules in antigen recognition by T cells. Keratinocytes can express Ia antigens in many inflammatory dermatoses and by action of gamma interferon (IFN-gamma). A keratinocyte cell line, Pam 212 cells, expressed Ia antigens at maintaining culture. They also expressed Ia antigens under the influence of IFN-gamma. Liposomes carrying molecules from trinitrophenylated-Pam cells did not stimulate lymphnode cells (LCs) of contact sensitivity; however, liposomes carrying a large amount of those molecules stimulated LCs. This stimulation was attributable to contaminated Ia antigens in those molecules. Liposomes with a small amount of those molecules or trinitrophenylated-proteins from Pam cells were prepared. Into them were inserted Ia antigen-rich proteins from Pam cells, spleen cells, IFN-gamma-treated Pam cells, or epidermal cells containing Langerhans cells. Thus prepared liposomes stimulated the LCs significantly. The Ia antigens obtained from IFN-gamma-treated Pam cells worked as well as Ia antigens from epidermal cells that contained Langerhans cells as the molecule of antigen presentation in contact sensitivity.
免疫反应相关（Ia）抗原作为T细胞识别抗原的分子非常重要。角质形成细胞在许多炎症性皮肤病中以及在γ干扰素（IFN-γ）的作用下可表达Ia抗原。一种角质形成细胞系，Pam 212细胞，在维持培养时表达Ia抗原。它们在IFN-γ的影响下也表达Ia抗原。携带来自三硝基苯化Pam细胞分子的脂质体不能刺激接触敏感性的淋巴结细胞（LCs）；然而，携带大量这些分子的脂质体可刺激LCs。这种刺激归因于那些分子中污染的Ia抗原。制备了含有少量来自Pam细胞的这些分子或三硝基苯化蛋白的脂质体。将来自Pam细胞、脾细胞、IFN-γ处理的Pam细胞或含有朗格汉斯细胞的表皮细胞中富含Ia抗原的蛋白质插入其中。如此制备的脂质体可显著刺激LCs。从IFN-γ处理的Pam细胞获得的Ia抗原与来自含有朗格汉斯细胞的表皮细胞的Ia抗原一样，可作为接触敏感性中抗原呈递的分子发挥作用。
