McCann F V, Keller T M, Guyre P M
J Membr Biol. 1987;96(1):57-64. doi: 10.1007/BF01869334.
The human cell line U-937 has been used extensively to model many macrophage functions. We have examined the cell membranes of human monocyte-derived macrophages (HMDM) and U-937 cells to compare membrane properties as expressed by single ion channel currents. The patch-clamp technique was applied to isolated, nonactivated, inside-out patches of cell membranes obtained from HMDM and from the U-937 cell line. Voltage-gated potassium channels of similar conductance but different kinetics are present in both types of cells, and a calcium-activated potassium channel is present only in the HMDM. These differences in ion channel properties suggest fundamentally different behavior between these two cell types at the level of the cell membrane.
人类细胞系U - 937已被广泛用于模拟多种巨噬细胞功能。我们检测了人单核细胞衍生巨噬细胞（HMDM）和U - 937细胞的细胞膜，以比较单离子通道电流所表现出的膜特性。膜片钳技术应用于从HMDM和U - 937细胞系分离得到的、未激活的、外翻式细胞膜片。两种细胞中均存在电导相似但动力学不同的电压门控钾通道，而钙激活钾通道仅存在于HMDM中。离子通道特性的这些差异表明这两种细胞类型在细胞膜水平上具有根本不同的行为。
