McCann F V, Cole J J, Guyre P M, Russell J A
Science. 1983 Feb 25;219(4587):991-3. doi: 10.1126/science.6823563.
The electrical activity of macrophages derived from human blood monocytes was recorded in vitro with intracellular microelectrodes and was analyzed with computer-assisted data acquisition and analysis techniques. In cells impaled 6 to 8 days after the cultures were prepared, the resting potentials reached a maximum value of -72 millivolts. The cells were electrically excitable; spikes exhibited a slow upstroke, a fast downstroke, a discrete threshold, a large overshoot, and a brief undershoot. Repetitive firing was induced by a maintained depolarizing current. A positive relation was observed between transmembrane currents and resting potential. Voltage-current relations were nonrectifying for subthreshold current injections. Since these cells had not been treated with any specific activation factors, the electrical activity recorded is evidence for the presence of voltage-dependent inward and outward currents in the membranes of mature macrophages. The electrical signals generated by these cells may be useful for the assay of sensor and effector functions of macrophages, such as chemotaxis, receptor-ligand interactions, and phagocytosis.
用人血单核细胞来源的巨噬细胞的电活动，通过细胞内微电极在体外进行记录，并采用计算机辅助数据采集和分析技术进行分析。在培养物制备后6至8天刺入的细胞中，静息电位达到-72毫伏的最大值。这些细胞具有电兴奋性；动作电位表现出缓慢的上升支、快速的下降支、离散的阈值、较大的超射和短暂的 undershoot。持续的去极化电流可诱导重复放电。观察到跨膜电流与静息电位之间呈正相关。对于阈下电流注入，电压-电流关系是非整流的。由于这些细胞未用任何特定的激活因子处理，所记录的电活动证明成熟巨噬细胞膜中存在电压依赖性内向和外向电流。这些细胞产生的电信号可能有助于检测巨噬细胞的传感器和效应器功能，如趋化性、受体-配体相互作用和吞噬作用。
