Department of Gynecology and Obstetrics, Women's Hospital School of Medicine Zhejiang University, Hangzhou, P. R. China.
J Microbiol. 2014 Jan;52(1):71-6. doi: 10.1007/s12275-014-3204-3. Epub 2014 Jan 4.
The genus Salmonella contains more than 2500 serovars. While most cause the self-limiting gastroenteritis, a few serovars can elicit typhoid fever, a severe systemic infection. S. enterica subsp. enterica serovar Typhimurium and S. Typhi are the representatives of the gastroenteritis and typhoid fever types of Salmonella. In this study, we adopted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) technology to quantitatively compare the proteomes of the two serovars. We found several proteins with serovar-specific expression, which could be developed as new biomarkers for clinical serotype diagnosis. We found that flagella and chemotaxis genes were down-regulated in S. Typhi in comparison with S. Typhimurium. We attributed this observation to the fact that the smooth cellular structure of S. Typhi may better fit its systemic lifestyle. Instead of known virulence factors that were located within Salmonella Pathogenecity Islands, a number of core genes, which were involved in metabolism and transport of carbohydrates and amino acids, showed differential expression between the two serovars. Further studies on the roles of these differentially-expressed genes in the pathogenesis should be undertaken.
沙门氏菌属包含超过 2500 个血清型。虽然大多数血清型引起自限性胃肠炎，但少数血清型可引起伤寒，一种严重的全身感染。肠道沙门氏菌亚种肠炎血清型鼠伤寒和伤寒沙门氏菌是胃肠炎和伤寒型沙门氏菌的代表。在这项研究中，我们采用了稳定同位素标记与氨基酸细胞培养技术（SILAC）对这两个血清型的蛋白质组进行定量比较。我们发现了一些具有血清型特异性表达的蛋白质，它们可以作为临床血清型诊断的新生物标志物。我们发现，与鼠伤寒沙门氏菌相比，伤寒沙门氏菌中的鞭毛和趋化性基因表达下调。我们将这一观察结果归因于伤寒沙门氏菌光滑的细胞结构可能更适合其全身生活方式。除了位于沙门氏菌致病性岛的已知毒力因子外，许多参与碳水化合物和氨基酸代谢和运输的核心基因在两个血清型之间表现出差异表达。应该对这些差异表达基因在发病机制中的作用进行进一步研究。
