Sun Danhua, Xu Jiwei
Faculty of Basic Medicine, Luohe Medical College, Luohe Henan 462002, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013 Oct;27(10):1225-9.
To investigate the effect of curcumin on calcitionin gene related peptide (CGRP) expression after spinal cord injury (SCI) in rats.
A total of 200 rats, weighing 250-300 g, were randomly divided into 4 groups (n = 50): sham-operation group, normal saline (NS) group, low-dose curcumin group (30 mg/kg), and high-dose curcumin group (100 mg/kg). In sham-operation group, only vertebral lamina excision was performed without SCI; the SCI model was established in the other 3 groups. At immediate after modeling, 30 mg/kg and 100 mg/kg curcumin were injected intraperitoneally in 2 curcumin groups, equivalent NS was given in NS group (30 mg/kg), but no treatment in sham-operation group. At 1, 3, 7, 14, and 21 days after operation, the motor neural function was evaluated by the inclined plane test and Basso-Beattie-Bresnahan (BBB) scores; immunohistochemical staining and Western blot assay were used to observe CGRP expression.
BBB score and inclined plane test score of NS group, low-dose curcumin group, and high-dose curcumin group were significantly lower than those of sham-operation group at each time point (P < 0.05). BBB score of low-dose curcumin group and high-dose curcumin group was significantly higher than that of NS group at 3, 7, 14, and 21 days after SCI (P < 0.05), and the score of high-dose group was significantly higher than that of low-dose curcumin group at 7, 14, and 21 days after SCI (P < 0.05). Inclined plane test score of low-dose curcumin group and high-dose curcumin group was significantly higher than that of NS group at 7, 14, and 21 days after SCI (P < 0.05), and the score of high-dose curcumin group was significantly higher than that of low-dose curcumin group at 7, 14, and 21 days after SCI (P < 0.05). Immunohistochemical staining results showed that the CGRP positive cells of sham-operation group was significantly more than those of the other 3 groups, and the CGRP positive cells of high-dose curcumin group were significantly more than those of low-dose curcumin group at each time point (P < 0.05); the CGRP positive cells of low- and high-dose curcumin groups were significantly more than those of NS group at 3, 7, 14, and 21 days after SCI (P < 0.05). Western blot assay results showed that the CGRP protein expressed at each time point after SCI in sham-operation group; the CGRP protein expression gradually decrease with time passing in NS group; but the CGRP protein expression gradually increased with time passing in low- and high-dose curcumin groups, and reached the peak at 14 days, then maintained a high level.
After SCI in rats, 30 mg/kg curcumin can improve rats' motor function, and 100 mg/kg curcumin effect is more obvious, especially in promoting the expression of CGRP. That may be the mechanism of protection of the nervous system.
探讨姜黄素对大鼠脊髓损伤（SCI）后降钙素基因相关肽（CGRP）表达的影响。
选取200只体重250 - 300 g的大鼠，随机分为4组（n = 50）：假手术组、生理盐水（NS）组、低剂量姜黄素组（30 mg/kg）和高剂量姜黄素组（100 mg/kg）。假手术组仅行椎板切除，不造成脊髓损伤；其余3组建立脊髓损伤模型。建模后即刻给2个姜黄素组分别腹腔注射30 mg/kg和100 mg/kg姜黄素，NS组给予等量生理盐水（30 mg/kg），假手术组不做处理。术后1、3、7、14和21天，通过斜板试验和Basso-Beattie-Bresnahan（BBB）评分评估运动神经功能；采用免疫组化染色及蛋白质印迹法观察CGRP表达情况。
NS组、低剂量姜黄素组和高剂量姜黄素组在各时间点的BBB评分和斜板试验评分均显著低于假手术组（P < 0.05）；脊髓损伤后3、7、14和21天，低剂量姜黄素组和高剂量姜黄素组的BBB评分显著高于NS组（P < 0.05），且高剂量组在脊髓损伤后7、14和21天的评分显著高于低剂量姜黄素组（P < 0.05）。脊髓损伤后7、14和21天，低剂量姜黄素组和高剂量姜黄素组的斜板试验评分显著高于NS组（P < 0.05），且高剂量姜黄素组在脊髓损伤后7、14和21天的评分显著高于低剂量姜黄素组（P < 0.05）。免疫组化染色结果显示，假手术组CGRP阳性细胞明显多于其他3组，且各时间点高剂量姜黄素组CGRP阳性细胞明显多于低剂量姜黄素组（P < 0.05）；脊髓损伤后3、7、14和21天，低、高剂量姜黄素组CGRP阳性细胞明显多于NS组（P < 0.05）。蛋白质印迹法检测结果显示，假手术组脊髓损伤后各时间点均有CGRP蛋白表达；NS组CGRP蛋白表达随时间逐渐降低；低、高剂量姜黄素组CGRP蛋白表达随时间逐渐升高，14天达到峰值，随后维持在较高水平。
大鼠脊髓损伤后，30 mg/kg姜黄素可改善大鼠运动功能，100 mg/kg姜黄素效果更明显，尤其在促进CGRP表达方面。这可能是其保护神经系统的机制。
