Institute of Pathogenic Biology, University of South China, Hengyang 421001, China.
Diagn Pathol. 2014 Jan 8;9:1. doi: 10.1186/1746-1596-9-1.
To provide the basis for further exploring the effect and its mechanism of Death domain associated protein (Daxx) on the progress of cervical carcinoma induced by human papillomavirus (HPV), the distribution and location of Daxx in cervical carcinoma with high risk HPV(HR-HPV) positive was analyzed.
The samples of normal cervical epithelial cells, cervical intraepithelial neoplasia grade I (CINI), CINII CINIII and cervical cancers were collected. Immunohistochemistry assay was used to analyze the distributions and locations of Daxx in the cervical tissue. Indirect immunoinfluorescence test was utilized to observe the locations of Daxx in Caski cells with HPV16 positive.
Under the light microscopy, the brown signals of Daxx distributed in the nuclei of normal cervical epithelial cells; Daxx mainly distributed in nuclear membrane and there were a small amount of Daxx in the nuclei in CINI. Daxx intensively distributed in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer. Under fluorescent microscopy, the distribution and location of Daxx in Caski cells was similarly to that in cervical cells of CINII, CINIII and cervical cancer.
In the progress of the cervical cancer, Daxx gradually translocates from nucleus into nuclear membrane, cytoplasm and cell membrane. Daxx locates in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer.
The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/4671548951113870.
为进一步探讨死亡结构域相关蛋白（Daxx）在人乳头瘤病毒（HPV）诱导的宫颈癌进展中的作用及其机制提供依据，分析高危型 HPV（HR-HPV）阳性宫颈癌中 Daxx 的分布和位置。
收集正常宫颈上皮细胞、宫颈上皮内瘤变 I 级（CINI）、CINII、CINIII 和宫颈癌组织标本。采用免疫组织化学法分析 Daxx 在宫颈组织中的分布和位置。采用间接免疫荧光试验观察 HPV16 阳性的 Caski 细胞中 Daxx 的位置。
在光镜下，Daxx 的棕色信号分布在正常宫颈上皮细胞的核内；Daxx 主要分布在核膜上，CINI 中有少量 Daxx 分布在核内。Daxx 在 CINII、CINIII 和宫颈癌中强烈分布在细胞质和细胞膜中。在荧光显微镜下，Caski 细胞中 Daxx 的分布和位置与 CINII、CINIII 和宫颈癌中的宫颈细胞相似。
在宫颈癌的进展过程中，Daxx 逐渐从核内转移到核膜、细胞质和细胞膜。Daxx 位于 CINII、CINIII 和宫颈癌的细胞质和细胞膜中。
