Consiglio per la ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C. G. Bertero 22, Rome 00156, Italy.
Viruses. 2014 Jan 6;6(1):106-36. doi: 10.3390/v6010106.
Next-generation high throughput sequencing technologies became available at the onset of the 21st century. They provide a highly efficient, rapid, and low cost DNA sequencing platform beyond the reach of the standard and traditional DNA sequencing technologies developed in the late 1970s. They are continually improved to become faster, more efficient and cheaper. They have been used in many fields of biology since 2004. In 2009, next-generation sequencing (NGS) technologies began to be applied to several areas of plant virology including virus/viroid genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. Identification and characterization of known and unknown viruses and/or viroids in infected plants are currently among the most successful applications of these technologies. It is expected that NGS will play very significant roles in many research and non-research areas of plant virology.
下一代高通量测序技术在 21 世纪初问世。它们提供了一种高效、快速和低成本的 DNA 测序平台，超越了 20 世纪 70 年代后期开发的标准和传统 DNA 测序技术。自 2004 年以来，它们一直在不断改进，变得更快、更高效、更便宜。自 2009 年以来，下一代测序（NGS）技术开始应用于植物病毒学的多个领域，包括病毒/类病毒基因组测序、发现和检测、生态学和流行病学、复制和转录。在感染植物中鉴定和描述已知和未知病毒和/或类病毒是这些技术目前最成功的应用之一。预计 NGS 将在植物病毒学的许多研究和非研究领域发挥非常重要的作用。
