Department of Gastroenterology of Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
Department of Gastroenterology of Shanghai Changning Center Hospital, Shanghai 200336, P.R. China.
Int J Oncol. 2014 Mar;44(3):849-57. doi: 10.3892/ijo.2014.2248. Epub 2014 Jan 8.
N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes β1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides (N-glycan) of cell proteins and the dysfunction of which is a common feature of various carcinomas. Nevertheless, the role of GnT-V remains controversial. Therefore, the clinical implication of GnT-V expression may differ in each cancer type. The implication of GnT-V status in patients with gastric cancer has not been studied extensively. In the present study, we examined GnT-V expression in gastric cancer specimen both at protein and mRNA levels. We compared GnT-V expression with clinical and pathologic variables. Kaplan-Meier survival curves were generated to show the cause-specific survival. Furthermore, the small interfering RNA was devised to downregulate the GnT-V mRNA expression in SGC7901 and BGC 823 cells. We characterized the function implication of GnT-V by cell proliferation and invasiveness analysis. Analysis in gastric cancer specimen revealed that GnT-V expression correlated with tumor grade and stage. The overall survival time of positive GnT-V expression in gastric cancer was significantly shorter than that of negative GnT-V expression. Moreover, the downregulation of GnT-V expression by small interfering RNA resulted in a decrease of cell proliferation and invasiveness in SGC7901 and BGC 823 cells accompanied by morphological change. This supports that GnT-V correlates with metastasis and prognosis in gastric cancer. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications.
N-乙酰氨基葡萄糖基转移酶 V（GnT-V）是一种酶，可催化天冬酰胺（N）-连接寡糖（N-聚糖）上 N-乙酰氨基葡萄糖的β1-6 分支，其功能障碍是各种癌的共同特征。然而，GnT-V 的作用仍存在争议。因此，GnT-V 表达的临床意义可能因每种癌症类型而异。胃癌患者中 GnT-V 状态的意义尚未得到广泛研究。在本研究中，我们在蛋白质和 mRNA 水平上检查了胃癌标本中的 GnT-V 表达。我们将 GnT-V 表达与临床和病理变量进行了比较。Kaplan-Meier 生存曲线用于显示特定原因的生存情况。此外，还设计了小干扰 RNA 下调 SGC7901 和 BGC823 细胞中的 GnT-V mRNA 表达。我们通过细胞增殖和侵袭性分析来描述 GnT-V 的功能意义。胃癌标本分析表明，GnT-V 表达与肿瘤分级和分期相关。胃癌中 GnT-V 表达阳性的总生存时间明显短于 GnT-V 表达阴性的总生存时间。此外，小干扰 RNA 下调 GnT-V 表达导致 SGC7901 和 BGC823 细胞的增殖和侵袭性降低，并伴有形态变化。这支持 GnT-V 与胃癌的转移和预后相关。这些结果有助于深入了解 GnT-V 在胃癌中的调控潜在的分子机制，并具有潜在的转化临床应用价值。
