Takahashi Toshiko, Hagisawa Shigeru, Yoshikawa Kazuyuki, Tezuka Fumiaki, Kaku Mitsuo, Ohyama Chikara
Department of Clinical Laboratory, Tohoku University Hospital, Hirosaki, Japan.
J Urol. 2006 Jan;175(1):90-3; discussion 93. doi: 10.1016/S0022-5347(05)00044-3.
GnT-V is an enzyme that catalyzes beta1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides of cell proteins. GnT-V expression has been closely related to malignant potentials in colon cancer, brain cancer and hepatocellular carcinoma. We determined whether GnT-V expression is predictive of superficial bladder cancer recurrence.
The cohort comprised 60 consecutive patients with first time superficial bladder cancer treated with transurethral resection. None of the patients received prophylactic intravesical therapy until recurrence. Paraffin embedded tumor specimens were immunohistochemically examined by the avidin-biotin peroxidase method using monoclonal antibody against GnT-V. Kaplan-Meier survival curves were generated to determine disease-free survival. Univariate and multivariate analyses were done to compare GnT-V expression to other clinical and pathological variables.
GnT-V expression correlated inversely with tumor grade and stage. The positive incidence of GnT-V in G1 to G3 tumors was 7 of 9 (78%), 21 of 43 (49%) and 3 of 8 (38%), respectively. GnT-V was positive in 26 of 44 cases of pTa (60%) and in 5 of 16 of pT1 (31%) disease. The 31 patients with positive GnT-V expression had significantly higher disease-free survival than the 29 with negative GnT-V expression (log rank test p = 0.0034). Multivariate analysis revealed that patient age, pT, grade and negative GnT-V expression were independent predictors of recurrence (p = 0.015, 0.001, 0.019 and 0.011, respectively).
Immunohistochemical detection of GnT-V is an independent predictor of superficial bladder cancer recurrence.
GnT-V是一种催化细胞蛋白天冬酰胺（N）连接寡糖上N-乙酰葡糖胺β1-6分支的酶。GnT-V的表达与结肠癌、脑癌和肝细胞癌的恶性潜能密切相关。我们确定GnT-V的表达是否可预测浅表性膀胱癌的复发。
该队列包括60例首次接受经尿道切除术治疗的浅表性膀胱癌患者。在复发之前，所有患者均未接受预防性膀胱内治疗。使用抗GnT-V单克隆抗体，通过抗生物素蛋白-生物素过氧化物酶法对石蜡包埋的肿瘤标本进行免疫组织化学检查。生成Kaplan-Meier生存曲线以确定无病生存期。进行单因素和多因素分析，以比较GnT-V表达与其他临床和病理变量。
GnT-V的表达与肿瘤分级和分期呈负相关。GnT-V在G1至G3肿瘤中的阳性发生率分别为9例中的7例（78%）、43例中的21例（49%）和8例中的3例（38%）。在44例pTa疾病患者中有26例（60%）GnT-V呈阳性，在16例pT1疾病患者中有5例（31%）呈阳性。GnT-V表达阳性的31例患者的无病生存期明显高于GnT-V表达阴性的29例患者（对数秩检验p = 0.0034）。多因素分析显示，患者年龄、pT、分级和GnT-V表达阴性是复发的独立预测因素（分别为p = 0.015、0.001、0.019和0.011）。
GnT-V的免疫组织化学检测是浅表性膀胱癌复发的独立预测因素。
