Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran.
Pharmacol Rep. 2013;65(5):1272-80. doi: 10.1016/s1734-1140(13)71485-3.
Crocin and safranal are the active substances of saffron and have many biological properties. In the present study, we compared the effects of crocin, safranal and diclofenac on local inflammation and its induced pain in rats.
Local inflammation was induced by intraplantar (ipl) injection of carrageenan (100 μl, 2%). Paw thickness was measured before and after carrageenan injection. Inflammatory pain responses including cold allodynia, mechanical allodynia and hyperalgesia were assessed using acetone spray and von Frey filament tests, respectively. The number of neutrophils in inflammatory zone was counted 6.5 h after injection of carrageenan.
Carrageenan produced edema, cold allodynia, mechanical allodynia and hyperalgesia and caused neutrophil infiltration in paw tissues. Crocin at doses of 25, 50 and 100 mg/kg, safranal at doses of 0.5, 1 and 2 mg/kg and diclofenac (as a reference drug) at a dose of 10 mg/kg attenuated edema, suppressed inflammatory pain responses and decreased the number of neutrophils.
The present study showed anti-inflammatory and antinociceptive activities for crocin, safranal and diclofenac in carrageenan model of local inflammation and inflammatory pain.
藏红花中的主要活性物质西红花苷和西红花醛具有多种生物学特性。本研究比较了西红花苷、西红花醛和双氯芬酸对大鼠局部炎症及其诱导疼痛的作用。
通过足跖内（ipl）注射角叉菜胶（100μl，2%）诱导局部炎症。在角叉菜胶注射前后测量足跖厚度。使用丙酮喷雾和 von Frey 细丝试验分别评估炎症性疼痛反应，包括冷感觉过敏、机械性感觉过敏和痛觉过敏。在注射角叉菜胶 6.5 小时后，计算炎症区中性粒细胞的数量。
角叉菜胶可引起水肿、冷感觉过敏、机械性感觉过敏和痛觉过敏，并导致足组织中的中性粒细胞浸润。25、50 和 100mg/kg 剂量的西红花苷、0.5、1 和 2mg/kg 剂量的西红花醛和 10mg/kg 剂量的双氯芬酸钠（作为参考药物）均可减轻水肿、抑制炎症性疼痛反应并减少中性粒细胞数量。
本研究表明西红花苷、西红花醛和双氯芬酸对角叉菜胶诱导的局部炎症和炎症性疼痛具有抗炎和镇痛作用。
