Palanisamy Marimuthu, Aldars-García Laila, Gil-Ramírez Alicia, Ruiz-Rodríguez Alejandro, Marín Francisco R, Reglero Guillermo, Soler-Rivas Cristina
Dept. of Production and Characterization of Novel Foods, CIAL-Research Inst. in Food Science (UAM+CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Biotechnol Prog. 2014 Mar-Apr;30(2):391-400. doi: 10.1002/btpr.1865. Epub 2014 Jan 22.
A pressurized water extraction (PWE) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. Extraction yields were higher in individual than sequential extractions being the optimal extraction parameters: 200°C, 5 cycles of 5 min each at 10.3 MPa. The crude polysaccharide (PSC) fractions, isolated from the PWE extracts contained mainly β-glucans (including chitooligosaccharides deriving from chitin hydrolysis), α-glucans, and other PSCs (hetero-/proteo-glucans) depending on the extraction temperature and mushroom strain considered. The observed bile acids-binding capacities of some extracts were similar to a β-glucan enriched fraction obtained from cereals.
为了从栽培蘑菇（双孢蘑菇、香菇和平菇）中提取具有胆汁酸结合能力的β-葡聚糖，开发了一种加压水提取（PWE）方法，以用作补充剂来设计具有降胆固醇特性的新型食品。单次提取的产率高于顺序提取，最佳提取参数为：200°C，在10.3 MPa下进行5个循环，每个循环5分钟。从PWE提取物中分离出的粗多糖（PSC）部分主要含有β-葡聚糖（包括来自几丁质水解的壳寡糖）、α-葡聚糖和其他PSC（杂/蛋白葡聚糖），这取决于所考虑的提取温度和蘑菇菌株。一些提取物观察到的胆汁酸结合能力与从谷物中获得的富含β-葡聚糖的部分相似。
