Mişe Yonar S, Yonar M E, Yöntürk Y, Pala A
Department of Aquaculture, Fisheries Faculty, Firat University, Elazig, Turkey.
J Anim Physiol Anim Nutr (Berl). 2014 Oct;98(5):936-41. doi: 10.1111/jpn.12162. Epub 2014 Jan 9.
In this study, effect of ellagic acid on some haematological, immunological and antioxidant parameters in the blood and various tissues of rainbow trout (Oncorhynchus mykiss) were examined. Four groups of rainbow trout were fed experimental diets containing either no ellagic acid (control) or supplemented with ellagic acid at 50 mg/kg diet (EA-50), 100 mg/kg diet (EA-100) or 150 mg/kg diet (EA-150) for 21 days. Samples of the blood and tissue (liver, kidney and spleen) were collected at the end of the experiment and analysed for their haematological profile (the red blood cell count, the haemoglobin concentration and the haematocrit level), immune response (the white blood cell count, the oxidative radical production (NBT activity), the total plasma protein and total immunoglobulin level) and oxidant/antioxidant status (the malondialdehyde level, the superoxide dismutase, catalase and glutathione peroxidase activity as well as the reduced glutathione concentration). The findings of this study demonstrated that ellagic acid had a positive effect on the haematological parameters, the immune response and the antioxidant enzyme activities of the fish.
在本研究中，检测了鞣花酸对虹鳟（Oncorhynchus mykiss）血液及各种组织中一些血液学、免疫学和抗氧化参数的影响。将四组虹鳟投喂含不同剂量鞣花酸的实验饲料，分别为不含鞣花酸（对照组）、添加50 mg/kg饲料的鞣花酸（EA - 50）、添加100 mg/kg饲料的鞣花酸（EA - 100）或添加150 mg/kg饲料的鞣花酸（EA - 150），持续21天。实验结束时采集血液和组织（肝脏、肾脏和脾脏）样本，分析其血液学指标（红细胞计数、血红蛋白浓度和血细胞比容水平）、免疫反应（白细胞计数、氧化自由基产生（NBT活性）、血浆总蛋白和总免疫球蛋白水平）以及氧化/抗氧化状态（丙二醛水平、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性以及还原型谷胱甘肽浓度）。本研究结果表明，鞣花酸对鱼类的血液学参数、免疫反应和抗氧化酶活性具有积极影响。
