Wang Hong, Wang Zhuyuan, Ye Minglang, Zong Shenfei, Li Mingyue, Chen Peng, Ma Xueqin, Cui Yiping
Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Advanced Photonics Center, Southeast University, Nanjing 210096, China.
Talanta. 2014 Feb;119:144-50. doi: 10.1016/j.talanta.2013.10.057. Epub 2013 Oct 30.
We construct a novel fluorescent, surface enhanced Raman scattering (SERS) encoded and magnetic nanoprobe for live cell imaging. To fabricate this nanoprobe, single walled carbon nanotube (SWNT) is used as the building scaffold while gold nanoparticles (Au NPs), superparamagnetic iron oxide nanoparticles (SPIONs) and quantum dots (QDs) are employed as the building blocks. Here, Au NPs serve as the SERS substrate and QDs act as the fluorescent agent. Au NPs and SPIONs are first adsorbed on the SWNT via electrostatic interactions. Then a silica layer is coated on the SWNT. Finally, QDs are attached on the silica shell. With such a structure, various optical signals can be readily encoded to the nanoprobe simply by using different Raman molecules and QDs with different emission wavelengths. Experimental results show that the as-prepared nanoprobe exhibits well fluorescence and SERS performance. Furthermore, in vitro experiments demonstrate that the nanoprobe can fulfill magnetic field guided fluorescence and SERS dual mode imaging of live cells. As a fascinating optical encoding material and a multifunctional nanoplatform, the presented nanoprobe holds genuine potential in future biosensing applications.
我们构建了一种用于活细胞成像的新型荧光、表面增强拉曼散射（SERS）编码磁性纳米探针。为制备这种纳米探针，单壁碳纳米管（SWNT）用作构建支架，而金纳米颗粒（Au NPs）、超顺磁性氧化铁纳米颗粒（SPIONs）和量子点（QDs）用作构建单元。在此，Au NPs用作SERS底物，QDs用作荧光剂。Au NPs和SPIONs首先通过静电相互作用吸附在SWNT上。然后在SWNT上包覆一层二氧化硅层。最后，QDs附着在二氧化硅壳上。通过这种结构，只需使用不同的拉曼分子和具有不同发射波长的QDs，就可以轻松地将各种光信号编码到纳米探针中。实验结果表明，所制备的纳米探针具有良好的荧光和SERS性能。此外，体外实验表明，该纳米探针能够实现活细胞的磁场引导荧光和SERS双模式成像。作为一种引人入胜的光学编码材料和多功能纳米平台，所展示的纳米探针在未来生物传感应用中具有真正的潜力。
