School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
Small. 2010 Jan;6(1):119-25. doi: 10.1002/smll.200901459.
In this study, surface-enhanced Raman spectroscopy (SERS)-encoded magnetic nanoparticles (NPs) are prepared and utilized as a multifunctional tagging material for cancer-cell targeting and separation. First, silver-embedded magnetic NPs are prepared, composed of an 18-nm magnetic core and a 16-nm-thick silica shell with silver NPs formed on the surface. After simple aromatic compounds are adsorbed on the silver-embedded magnetic NPs, they are coated with silica to provide them with chemical and physical stability. The resulting silica-encapsulated magnetic NPs (M-SERS dots) produce strong SERS signals and have magnetic properties. In a model application as a tagging material, the M-SERS dots are successfully utilized for targeting breast-cancer cells (SKBR3) and floating leukemia cells (SP2/O). The targeted cancer cells can be easily separated from the untargeted cells using an external magnetic field. The separated targeted cancer cells exhibit a Raman signal originating from the M-SERS dots. This system proves to be an efficient tool for separating targeted cells. Additionally, the magnetic-field-induced hot spots, which can provide a 1000-times-stronger SERS intensity due to aggregation of the NPs, are studied.
在本研究中，制备了表面增强拉曼光谱（SERS）编码磁性纳米粒子（NPs），并将其用作癌症细胞靶向和分离的多功能标记材料。首先，制备了嵌入银的磁性 NPs，由 18nm 的磁性核和 16nm 厚的二氧化硅壳组成，表面形成了银 NPs。简单的芳香族化合物被吸附在嵌入银的磁性 NPs 上之后，它们被涂覆上二氧化硅以提供化学和物理稳定性。所得的二氧化硅包覆的磁性 NPs（M-SERS 点）产生强的 SERS 信号并具有磁性。在作为标记材料的模型应用中，M-SERS 点成功地用于靶向乳腺癌细胞（SKBR3）和漂浮白血病细胞（SP2/O）。使用外部磁场可以轻松地将靶向癌细胞与非靶向细胞分离。分离的靶向癌细胞表现出源自 M-SERS 点的拉曼信号。该系统被证明是一种分离靶向细胞的有效工具。此外，还研究了磁场诱导的热点，由于 NPs 的聚集，热点可以提供 1000 倍强的 SERS 强度。
