Fang Biyun, Liang Yan, Chen Fang
School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Talanta. 2014 Feb;119:601-5. doi: 10.1016/j.talanta.2013.11.066. Epub 2013 Dec 1.
A sensitive and selective copper(II) fluorescence Schiff base chemical sensor receptor 1 (short for N,N'-bis(salicylidene)-o-phenylenediamine) has been prepared. The fluorescence of receptor 1 in pH 8.2 phosphate buffer solution can be dramatically quenched by Cu(2+), whereas it is nearly unaffected by other metal ions. Based on this, a sensitive and selective fluorescent quenching method for Cu(2+) detection has been established. Under the optimum conditions, a good linear relation exists between the quenching efficiency (F0/F) and the concentration of Cu(2+) in the range of 1.0 × 10(-7)-2.5 × 10(-6) mol L(-1). The detection limit (3σ) for Cu(2+) determination is 2.0 × 10(-8) mol L(-1). The present method has been successfully used for quantification of Cu(2+) in soybean milk powder. Furthermore, the fluorescence switch property of the system was explored, and the system might be applied for determination of glutathione and construction of molecular logic gate.
一种灵敏且具有选择性的铜（II）荧光席夫碱化学传感器受体1（即N，N'-双（水杨醛）-邻苯二胺的简称）已被制备出来。受体1在pH 8.2的磷酸盐缓冲溶液中的荧光可被Cu（2+）显著猝灭，而其他金属离子对其几乎没有影响。基于此，建立了一种灵敏且具有选择性的用于检测Cu（2+）的荧光猝灭方法。在最佳条件下，猝灭效率（F0/F）与Cu（2+）浓度在1.0×10（-7）-2.5×10（-6）mol L（-1）范围内存在良好的线性关系。测定Cu（2+）的检测限（3σ）为2.0×10（-8）mol L（-1）。该方法已成功用于豆浆粉中Cu（2+）的定量分析。此外，还探索了该体系的荧光开关特性，该体系可能用于谷胱甘肽的测定及分子逻辑门的构建。
