Xu Canhui, Liao Lifu, He Yunfei, Wu Rurong, Li Shijun, Yang Yanyan
College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2015;149:662-6. doi: 10.1016/j.saa.2015.04.103. Epub 2015 May 8.
A Schiff base-type fluorescence probe was prepared for the detection of copper (II) in foodstuffs. The probe is N,N'-bis(pyridoxal phosphate)-o-phenylenediamine (BPPP). It was synthesized by utilizing the Schiff base condensation reaction of pyridoxal 5-phosphate with 1,2-phenylenediamine. BPPP has the properties of high fluorescence stability, good water solubility and low toxicity. Its maximum excitation wavelength and maximum fluorescence emission wavelength are at 389 and 448 nm, respectively. When BPPP coexists with copper (II), its fluorescence is dramatically quenched. Under a certain condition, the fluorescence intensity decreased proportionally to the concentration of copper (II) by the quenching effect. Based on this fact, we established a fluorescence quenching method for the determination of copper (II). Under optimal conditions a linear range was found to be 0.5-50 ng/mL with a detection limit of 0.2 ng/mL. The method has been applied to determine copper (II) in foodstuff samples and the analytical results show good agreement with that obtained from atomic absorption spectrometry method.
制备了一种席夫碱型荧光探针用于检测食品中的铜（II）。该探针为N,N'-双（磷酸吡哆醛）-邻苯二胺（BPPP）。它是通过5-磷酸吡哆醛与1,2-苯二胺的席夫碱缩合反应合成的。BPPP具有荧光稳定性高、水溶性好和低毒性的特性。其最大激发波长和最大荧光发射波长分别为389 nm和448 nm。当BPPP与铜（II）共存时，其荧光会显著猝灭。在一定条件下，由于猝灭效应，荧光强度与铜（II）的浓度成比例降低。基于这一事实，我们建立了一种测定铜（II）的荧光猝灭方法。在最佳条件下，线性范围为0.5 - 50 ng/mL，检测限为0.2 ng/mL。该方法已应用于食品样品中铜（II）的测定，分析结果与原子吸收光谱法获得的结果吻合良好。
