Billa Nanditha, Hubin-Barrows Dylan, Lahren Tylor, Burkhard Lawrence P
Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA.
Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA.
Talanta. 2014 Feb;119:620-2. doi: 10.1016/j.talanta.2013.09.051. Epub 2013 Oct 21.
Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [2] and recently validated for small samples by Inouye and Lotufo (2006) [1]. With the accelerated solvent extraction method using chloroform:methanol solvent and the colorimetric lipid determination method, 28 of 30 samples had significant proportional bias (α=1%, determined using standard additions) and 1 of 30 samples had significant constant bias (α=1%, determined using Youden Blank measurements). With sonic extraction, 0 of 6 samples had significant proportional bias (α=1%) and 1 of 6 samples had significant constant bias (α=1%). These demonstrate that the accelerated solvent extraction method with chloroform:methanol solvent system creates an interference with the colorimetric assay method, and without accounting for the bias in the analysis, inaccurate measurements would be obtained.
对两种常见的实验室提取技术进行了评估，以便与Van Handel（1985年）[2]开发并由Inouye和Lotufo（2006年）[1]最近针对小样本验证的微量比色法脂质测定方法常规使用。采用氯仿：甲醇溶剂的加速溶剂萃取法和比色法脂质测定法，30个样本中有28个存在显著比例偏差（α = 1%，使用标准加入法测定），30个样本中有1个存在显著恒定偏差（α = 1%，使用尤登空白测量法测定）。采用超声萃取时，6个样本中0个存在显著比例偏差（α = 1%），6个样本中有1个存在显著恒定偏差（α = 1%）。这些结果表明，氯仿：甲醇溶剂体系的加速溶剂萃取法对比色测定法产生了干扰，并且如果不考虑分析中的偏差，将会获得不准确的测量结果。
