Department of Biotechnology, Instituto Tecnológico de Canarias, Pozo Izquierdo s/n (35119), Santa Lucía de Tirajana, Las Palmas, Canary Islands, Spain.
Department of Biotechnology, Instituto Tecnológico de Canarias, Pozo Izquierdo s/n (35119), Santa Lucía de Tirajana, Las Palmas, Canary Islands, Spain.
Bioresour Technol. 2015 Dec;197:369-74. doi: 10.1016/j.biortech.2015.07.094. Epub 2015 Jul 29.
The lipid extractability of 14 microalgae species and strains was assessed using organic solvents (methanol and chloroform). The high variability detected indicated the potential for applying this parameter as an additional criterion for microalgae screening in industrial processes such as biofuel production from microalgae. Species without cell walls presented higher extractability than species with cell walls. Analysis of cell integrity by flow cytometry and staining with propidium iodide showed a significant correlation between higher resistance to the physical treatments of cell rupture by sonication and the lipid extractability of the microalgae. The results highlight the cell wall as a determining factor in the inter- and intraspecific variability in lipid extraction treatments.
采用有机溶剂（甲醇和氯仿）评估了 14 种微藻物种和品系的脂质可提取性。检测到的高变异性表明，该参数有可能作为微藻筛选的附加标准，应用于微藻生物燃料生产等工业过程。无细胞壁的物种比有细胞壁的物种具有更高的可提取性。通过流式细胞术分析细胞完整性并用碘化丙啶染色表明，在超声细胞破裂的物理处理方面具有更高的抗性与微藻的脂质可提取性之间存在显著相关性。结果突出了细胞壁作为脂质提取处理中种间和种内变异性的决定因素。
