Sun Ze-lin, Zhang Ya-zhuo
Department of Neurosurgery, Hebei United University Affiliated Hospital, Tangshan 063000, China.
Beijing Neurosurgical Institute,Beijing 100050, China,Email:
Zhonghua Yi Xue Za Zhi. 2013 Nov 5;93(41):3306-8.
To explore the relationship between multi-differentiation potential of monoclonal immortalized human mesenchymal stem cells (hMSC-TERT) in vivo and their cluster of differentiation antigens (CD).
Monoclonal hMSC-TERT were isolated using limiting dilution. Direct immunofluorescence staining flow cytometry was used to detect the cluster of differentiation antigens (CD44, CD45, CD105) of these cell lines. Their adipocytic, osteogenic, neuronal differentiation potential in vitro were determined by Oil Red O staining, Von Kossa staining and immunocytochemistry for tubulin-β III antibody. Then hMSC-TERTs were transplanted subcutaneously into severe combined immunodeficiency (SCID) mice. The cell grafts were removed and analyzed by immunohistochemistry for pathologic tissue markers for multi-differentiation potential of hMSC-TERT cells in vivo.
CD105+ cell was 84.68% positive in hMSC-TERT-C19 while <5% in other monoclonal cell lines. The positive rate of cytokeratin in grafts formed by hMSC-TERT-C19 cell line in SCID mice was much higher than the others (P < 0.01).
The positive CD105 of monoclonal hMSC-TERTs may be directly correlated with its epithelial differentiation potential.
探讨单克隆永生化人骨髓间充质干细胞（hMSC-TERT）在体内的多分化潜能与其分化抗原簇（CD）之间的关系。
采用有限稀释法分离单克隆hMSC-TERT。运用直接免疫荧光染色流式细胞术检测这些细胞系的分化抗原簇（CD44、CD45、CD105）。通过油红O染色、冯科萨染色以及针对微管蛋白-β III抗体的免疫细胞化学方法，测定它们在体外的脂肪形成、成骨、神经元分化潜能。随后将hMSC-TERTs皮下移植到严重联合免疫缺陷（SCID）小鼠体内。取出细胞移植物，通过免疫组织化学分析hMSC-TERT细胞在体内多分化潜能的病理组织标志物。
hMSC-TERT-C19中CD105+细胞阳性率为84.68%，而其他单克隆细胞系中该阳性率<5%。hMSC-TERT-C19细胞系在SCID小鼠体内形成的移植物中细胞角蛋白阳性率远高于其他细胞系（P < 0.01）。
单克隆hMSC-TERTs的CD105阳性可能与其上皮分化潜能直接相关。
